Detailed information NTG0392037

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GA645_RS15540
• Genome accession: NZ_CP045118
• Coordinates: 3385508..3386725 (-)
• Length: 405 a.a.
• NCBI ID: WP_152223912.1
• Uniprot ID: -
• Organism: Pseudomonas sp. SCB32
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3380508..3391725

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GA645_RS15520	-	3381433..3382875 (-)	1443	WP_152223910.1	sodium:alanine symporter family protein	-
GA645_RS15525	-	3383044..3383862 (+)	819	WP_152223911.1	helix-turn-helix transcriptional regulator	-
GA645_RS15530	-	3384017..3384901 (-)	885	WP_152228124.1	DMT family transporter	-
GA645_RS15540	pilU	3385508..3386725 (-)	1218	WP_152223912.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GA645_RS15545	-	3386914..3387192 (+)	279	WP_152223913.1	peptidylprolyl isomerase	-
GA645_RS15550	-	3387200..3387565 (+)	366	WP_152223914.1	MmcQ/YjbR family DNA-binding protein	-
GA645_RS15555	-	3387562..3388116 (+)	555	WP_152223915.1	putative glycolipid-binding domain-containing protein	-
GA645_RS15560	-	3388440..3389621 (+)	1182	WP_152223916.1	MFS transporter	-
GA645_RS15565	-	3389947..3391224 (+)	1278	WP_152223917.1	OprD family porin	-

Sequence

Protein

• Length: 405 a.a.
• Molecular weight: 44338.53 Da
• Isoelectric Point: 5.9206

>NTDB_id=392037 GA645_RS15540 WP_152223912.1 3385508..3386725(-) (pilU) [Pseudomonas sp. SCB32]
MDIQSMLKILATQDGSDLYLSTGAPPCAKFNGVLKPLSNEPLKSGDVARIGYGLMDEEQRADFERELEMNLAISVQGVGR
FRINLFKQRNEVSIVARNIKLDIPLFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDIIKRGDFSSIKEIMDKSRALGMQTFDQAL
FDLAVEGAISEDEAVKNADSANNLRLKLKLYKDNPAMVTNSAAAAAPAPASVAAAAPVTEASSWGLELALEEIEAEDAES
KQAGQ

Nucleotide

• Length: 1218 bp

>NTDB_id=392037 GA645_RS15540 WP_152223912.1 3385508..3386725(-) (pilU) [Pseudomonas sp. SCB32]
ATGGATATCCAGTCGATGCTGAAGATCCTGGCCACCCAGGATGGTTCCGACCTCTACCTTTCCACCGGCGCGCCGCCCTG
TGCCAAGTTCAACGGGGTCCTCAAGCCGCTGTCCAACGAACCGCTCAAGAGCGGTGACGTGGCGCGCATCGGCTACGGCC
TGATGGATGAGGAGCAGCGTGCCGATTTTGAGCGCGAACTGGAGATGAACCTGGCCATTTCGGTGCAGGGCGTGGGCCGT
TTCCGTATCAACCTGTTCAAGCAGCGCAACGAAGTGTCCATCGTGGCGCGTAACATCAAGCTGGACATTCCGCTGTTCGA
AGACCTGAAGTTGCCGGAAGTGCTGCTCAAGGTCGTCATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGTACCGGCT
CGGGCAAGTCCACTTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAC
CCGATCGAGTACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGCGAAGTGGGCGTGGACACCCGCAGCTTCCATGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCGATCTCCACCTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGGCCGCAACTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAGCG
CCTGGTACGCACCCAGGACGGCAAGCGTCGCGCCGCGGTGGAAGTGCTGCTGGGCACGGCGACCATCAGCGACATCATCA
AGCGCGGTGACTTCAGCTCGATCAAGGAAATCATGGACAAGTCCCGCGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGCGGTGGAAGGGGCGATCAGTGAAGACGAAGCGGTGAAGAACGCCGACTCGGCGAACAACCTGCGTCTGAA
ACTCAAGCTCTACAAGGACAACCCGGCCATGGTGACCAACTCCGCGGCAGCTGCCGCCCCCGCGCCGGCGTCCGTTGCTG
CGGCGGCTCCGGTGACGGAAGCCTCCAGCTGGGGGTTGGAGCTGGCACTGGAAGAGATCGAGGCCGAGGACGCGGAGAGC
AAGCAGGCCGGGCAATAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.125	86.667	0.486
pilU	Acinetobacter baylyi ADP1	53.409	86.914	0.464
pilU	Vibrio cholerae strain A1552	51.714	86.42	0.447
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.529	94.321	0.373