Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   ASTEX_RS01515 Genome accession   NC_014816
Coordinates   332645..333127 (-) Length   160 a.a.
NCBI ID   WP_013477840.1    Uniprot ID   E8RPM9
Organism   Asticcacaulis excentricus CB 48     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 324506..356328 332645..333127 within 0


Gene organization within MGE regions


Location: 324506..356328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASTEX_RS01445 (Astex_0295) - 324506..325738 (-) 1233 WP_013477827.1 divergent polysaccharide deacetylase family protein -
  ASTEX_RS01450 (Astex_0296) - 325982..326419 (-) 438 WP_013477828.1 hypothetical protein -
  ASTEX_RS01455 (Astex_0297) - 326400..326879 (-) 480 WP_013477829.1 Panacea domain-containing protein -
  ASTEX_RS01460 (Astex_0298) - 327309..327704 (+) 396 WP_013477830.1 hypothetical protein -
  ASTEX_RS01465 (Astex_0299) - 327697..327996 (+) 300 WP_013477831.1 hypothetical protein -
  ASTEX_RS01470 (Astex_0300) - 328019..329089 (-) 1071 WP_013477832.1 tyrosine-type recombinase/integrase -
  ASTEX_RS01475 (Astex_0301) - 329071..329322 (-) 252 WP_013477833.1 hypothetical protein -
  ASTEX_RS01480 (Astex_0302) - 329319..329708 (-) 390 WP_013477834.1 hypothetical protein -
  ASTEX_RS01485 (Astex_0303) - 329705..330382 (-) 678 WP_013477835.1 hypothetical protein -
  ASTEX_RS01490 (Astex_0304) - 330382..330933 (-) 552 WP_013477836.1 hypothetical protein -
  ASTEX_RS01495 (Astex_0305) - 330923..331459 (-) 537 WP_013477837.1 hypothetical protein -
  ASTEX_RS19140 (Astex_0306) - 331456..332007 (-) 552 WP_013477838.1 hypothetical protein -
  ASTEX_RS01510 (Astex_0307) - 332009..332293 (-) 285 WP_013477839.1 hypothetical protein -
  ASTEX_RS01515 (Astex_0308) ssb 332645..333127 (-) 483 WP_013477840.1 single-stranded DNA-binding protein Machinery gene
  ASTEX_RS01520 (Astex_0310) - 333228..333539 (-) 312 WP_013477842.1 hypothetical protein -
  ASTEX_RS19145 (Astex_0311) - 333542..333874 (-) 333 WP_083805599.1 DUF2312 domain-containing protein -
  ASTEX_RS01530 (Astex_0312) - 334146..334421 (-) 276 WP_013477844.1 hypothetical protein -
  ASTEX_RS19735 (Astex_0316) - 335061..335753 (-) 693 WP_144004583.1 S24 family peptidase -
  ASTEX_RS20215 (Astex_0317) - 336095..336676 (+) 582 WP_013477849.1 hypothetical protein -
  ASTEX_RS01555 (Astex_0318) - 336673..337263 (+) 591 WP_013477850.1 hypothetical protein -
  ASTEX_RS01560 (Astex_0319) - 337260..337709 (+) 450 WP_013477851.1 phage regulatory CII family protein -
  ASTEX_RS01565 (Astex_0320) - 337706..338449 (+) 744 WP_013477852.1 GcrA cell cycle regulator -
  ASTEX_RS01570 (Astex_0321) - 338439..338768 (+) 330 WP_013477853.1 hypothetical protein -
  ASTEX_RS01575 (Astex_0322) - 338761..339468 (+) 708 WP_144004584.1 hypothetical protein -
  ASTEX_RS01580 (Astex_0323) - 339465..340634 (+) 1170 WP_013477855.1 hypothetical protein -
  ASTEX_RS01585 (Astex_0324) - 340631..342196 (+) 1566 WP_013477856.1 phage/plasmid primase, P4 family -
  ASTEX_RS01590 (Astex_0325) - 342485..343021 (+) 537 WP_013477857.1 ngn domain-containing protein -
  ASTEX_RS01595 (Astex_0326) - 343347..343646 (+) 300 WP_013477858.1 HNH endonuclease -
  ASTEX_RS01600 (Astex_0327) - 343821..344318 (+) 498 WP_013477859.1 hypothetical protein -
  ASTEX_RS01605 (Astex_0328) - 344323..346011 (+) 1689 WP_013477860.1 terminase TerL endonuclease subunit -
  ASTEX_RS01610 (Astex_0329) - 346021..347259 (+) 1239 WP_013477861.1 phage portal protein -
  ASTEX_RS01615 (Astex_0330) - 347231..348055 (+) 825 WP_013477862.1 head maturation protease, ClpP-related -
  ASTEX_RS01620 (Astex_0331) - 348083..349384 (+) 1302 WP_013477863.1 phage major capsid protein -
  ASTEX_RS01625 (Astex_0332) - 349467..349904 (+) 438 WP_013477864.1 hypothetical protein -
  ASTEX_RS01630 (Astex_0333) - 349978..350283 (+) 306 WP_013477865.1 head-tail connector protein -
  ASTEX_RS01635 (Astex_0334) - 350294..350479 (+) 186 WP_013477866.1 hypothetical protein -
  ASTEX_RS01640 (Astex_0335) - 350479..350817 (+) 339 WP_013477867.1 head-tail adaptor protein -
  ASTEX_RS01645 (Astex_0336) - 350814..351230 (+) 417 WP_013477868.1 hypothetical protein -
  ASTEX_RS19155 (Astex_0337) - 351234..351656 (+) 423 WP_013477869.1 DUF3168 domain-containing protein -
  ASTEX_RS01655 (Astex_0338) - 351729..352196 (+) 468 WP_013477870.1 phage tail tube protein -
  ASTEX_RS01660 (Astex_0339) - 352196..352549 (+) 354 WP_013477871.1 gene transfer agent family protein -
  ASTEX_RS20220 (Astex_0340) - 352561..352761 (+) 201 WP_041658415.1 hypothetical protein -
  ASTEX_RS01670 (Astex_0341) - 352772..355723 (+) 2952 WP_013477873.1 tape measure protein -
  ASTEX_RS01675 (Astex_0342) - 355720..356328 (+) 609 WP_013477874.1 hypothetical protein -

Sequence


Protein


Download         Length: 160 a.a.        Molecular weight: 17713.78 Da        Isoelectric Point: 5.8285

>NTDB_id=39199 ASTEX_RS01515 WP_013477840.1 332645..333127(-) (ssb) [Asticcacaulis excentricus CB 48]
MAGSVNKVIIIGHLGKDPEIRTTNSGDRIANMTVATSETWRDKLSGDRKEKTEWHRVVIFNDALAKIAEEYLRKGSQVYL
EGSLATRKWTDQQGVEKYTTEIVLQRFNGTLTLLGGRGEGKGPPAAQSQDEYGFDRPATRGVDATGRSVEPSPFDDEIPF

Nucleotide


Download         Length: 483 bp        

>NTDB_id=39199 ASTEX_RS01515 WP_013477840.1 332645..333127(-) (ssb) [Asticcacaulis excentricus CB 48]
ATGGCGGGATCCGTCAACAAGGTCATCATCATCGGCCACCTTGGGAAAGATCCCGAGATCCGCACCACGAACTCGGGCGA
TCGCATCGCCAACATGACCGTCGCCACGTCTGAGACATGGCGGGACAAGTTGTCTGGCGACCGCAAGGAAAAGACCGAAT
GGCATCGCGTGGTCATCTTCAATGACGCCCTTGCGAAGATTGCCGAGGAGTACCTGCGCAAGGGCTCACAGGTATATCTC
GAAGGCTCACTCGCAACGCGGAAGTGGACCGATCAGCAGGGCGTCGAGAAGTACACGACTGAGATCGTACTGCAGCGGTT
CAATGGCACTCTGACCCTGCTTGGGGGCCGTGGCGAGGGCAAAGGCCCACCCGCCGCTCAGAGCCAGGACGAATACGGCT
TCGATCGCCCGGCAACCCGTGGCGTGGACGCCACCGGACGCAGCGTTGAGCCCTCCCCCTTCGATGATGAAATTCCGTTT
TGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB E8RPM9

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

47.778

100

0.538

  ssb Glaesserella parasuis strain SC1401

45.055

100

0.513

  ssb Neisseria meningitidis MC58

39.227

100

0.444

  ssb Neisseria gonorrhoeae MS11

39.227

100

0.444


Multiple sequence alignment