Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GBN68_RS15045 Genome accession   NZ_CP045101
Coordinates   3076582..3077304 (-) Length   240 a.a.
NCBI ID   WP_234954454.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ210     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3071582..3082304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN68_RS15025 (GBN68_15035) aroB 3072264..3073349 (-) 1086 WP_234954450.1 3-dehydroquinate synthase -
  GBN68_RS15030 (GBN68_15040) - 3073380..3073862 (-) 483 WP_207778853.1 shikimate kinase -
  GBN68_RS15035 (GBN68_15045) pilQ 3073951..3076035 (-) 2085 WP_234954452.1 type IV pilus secretin PilQ family protein Machinery gene
  GBN68_RS15040 (GBN68_15050) pilP 3076055..3076582 (-) 528 WP_094148569.1 pilus assembly protein PilP Machinery gene
  GBN68_RS15045 (GBN68_15055) pilO 3076582..3077304 (-) 723 WP_234954454.1 type 4a pilus biogenesis protein PilO Machinery gene
  GBN68_RS15050 (GBN68_15060) pilN 3077301..3077957 (-) 657 WP_184117975.1 PilN domain-containing protein Machinery gene
  GBN68_RS15055 (GBN68_15065) pilM 3077957..3079015 (-) 1059 WP_058870420.1 pilus assembly protein PilM Machinery gene
  GBN68_RS15060 (GBN68_15070) ponA 3079182..3081710 (+) 2529 WP_234954456.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 240 a.a.        Molecular weight: 27018.45 Da        Isoelectric Point: 4.9937

>NTDB_id=391927 GBN68_RS15045 WP_234954454.1 3076582..3077304(-) (pilO) [Acinetobacter johnsonii strain AJ210]
MSRDEFEELTSENHAVTKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNAGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNEKKTDIPQISYSVKAKTYRYVGATAAASVENTTKADAGGAQ

Nucleotide


Download         Length: 723 bp        

>NTDB_id=391927 GBN68_RS15045 WP_234954454.1 3076582..3077304(-) (pilO) [Acinetobacter johnsonii strain AJ210]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCACGCTGTAACCAAGAAAAAAATGACGGTTGATAAGTTTTT
TCAACAGTTTAATACCTTAGATCCAAACAATTACGGTAGTTGGCCCTTTTCAGTCAAATTGACTTGTTGGATCTTTATCA
TTTTAGTGATTGGGGCATTGGGTTATTTTGTCGTGATTAAGCCGAAGCTTGACGCGATTTCAAATGCACATGCACAAGAA
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAACTTGCAGCAATATCAAGCGCAATTGCAAGAAAT
GGAAGCCAATTTCAATCAGCAGTTAGAGCAGTTGCCGAAAGAAACTGAAATTCCTGGTTTGGTTGAAGATATCAACGTGA
GCGGCGTAAATGCTGGATTAAAGTTTAAAAATATTCGTTTAGAGCCAGAAATTCGCCAAGAGTTCTTTATCGAACAGCCG
ATTTCTATTGAAGCGACGGGTGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTGCCGCGTATTGTGAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAATAATGAGAAAAAGACGGATATTCCGCAAATTTCATACAGTGTAAAGG
CTAAAACTTACCGCTATGTCGGGGCGACAGCTGCTGCAAGTGTGGAAAATACAACTAAAGCAGATGCAGGAGGTGCTCAG
TAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

71.967

99.583

0.717
  comO Acinetobacter baylyi ADP1

68.033

100

0.692

Multiple sequence alignment