Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GBN68_RS15035 Genome accession   NZ_CP045101
Coordinates   3073951..3076035 (-) Length   694 a.a.
NCBI ID   WP_234954452.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ210     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3068951..3081035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN68_RS15020 (GBN68_15030) - 3071417..3072256 (-) 840 WP_234954448.1 hypothetical protein -
  GBN68_RS15025 (GBN68_15035) aroB 3072264..3073349 (-) 1086 WP_234954450.1 3-dehydroquinate synthase -
  GBN68_RS15030 (GBN68_15040) - 3073380..3073862 (-) 483 WP_207778853.1 shikimate kinase -
  GBN68_RS15035 (GBN68_15045) pilQ 3073951..3076035 (-) 2085 WP_234954452.1 type IV pilus secretin PilQ family protein Machinery gene
  GBN68_RS15040 (GBN68_15050) pilP 3076055..3076582 (-) 528 WP_094148569.1 pilus assembly protein PilP Machinery gene
  GBN68_RS15045 (GBN68_15055) pilO 3076582..3077304 (-) 723 WP_234954454.1 type 4a pilus biogenesis protein PilO Machinery gene
  GBN68_RS15050 (GBN68_15060) pilN 3077301..3077957 (-) 657 WP_184117975.1 PilN domain-containing protein Machinery gene
  GBN68_RS15055 (GBN68_15065) pilM 3077957..3079015 (-) 1059 WP_058870420.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 694 a.a.        Molecular weight: 75410.61 Da        Isoelectric Point: 5.2466

>NTDB_id=391925 GBN68_RS15035 WP_234954452.1 3073951..3076035(-) (pilQ) [Acinetobacter johnsonii strain AJ210]
MKNSAKDFIFGDGNTMNHMFRQFSMGAIAMAVMQAASAQVSITNIVPMQIPGQGTEIRVMFNGLPPQPQAYQLEQPSRLV
LDFDKAQQSLKQGSIAVATAEASTVDVTSDAQRSRLTVNLTDAGAFTTRVEGNTFILKINSANPMVATPVAAARSEQGIS
NIGFQRGSGGEGQIVIDLKGSNTPVDVKQQGSKIVVRVLGNKIPAHLSRRLNVNDFATPVSTIDAQNDGSNGLITIQSNG
SFEYMAYQAEDKLTISLKRPEDKNPMKPKAPSAYTGKKISLDFQDIEVRRVLQLLADFTGINMVASDTVQGNITLRLKDV
PWDQALDIIMKTKNLDKRRNGNVIWIAPVAELIKSEEDEAKAIAQSIKLSPIQTDYIQLSYAKSADVLKLLEDSRDSKGS
ESNRTAGSDSLALESLLSARGSAVSDSRTNTLIINDTQQNIDKIRRMIDLLDVAVKQVMVEARIVTASTDFSRELGIKWS
AKKEAGNNSPTWGGDFQPSFDLGVDLATSTAGTFNFGLLKISDYMLNLELTALQADGHGEVLSAPKVLTGDKQKAYILRG
TEIPYQTWSATEGLKTEFRAANLKLEVTPSITPDGKVQMALMVEKDSIGDITNDGIAIDTSELQTNVLVADGETVVLGGI
FDDVKRNDYQKVPFLGDLPVVGNLFKNNTKTTMQTELLIFVTPRIVNDSVSRNH

Nucleotide


Download         Length: 2085 bp        

>NTDB_id=391925 GBN68_RS15035 WP_234954452.1 3073951..3076035(-) (pilQ) [Acinetobacter johnsonii strain AJ210]
ATGAAAAATAGTGCTAAAGATTTTATATTTGGGGATGGCAATACGATGAACCATATGTTCCGTCAGTTTTCAATGGGTGC
AATTGCAATGGCGGTGATGCAGGCTGCAAGTGCGCAAGTCAGTATTACGAATATTGTGCCGATGCAAATTCCGGGACAGG
GAACAGAAATTCGTGTCATGTTCAACGGTTTGCCACCACAGCCGCAAGCCTATCAGTTGGAACAGCCATCACGTTTGGTT
TTGGATTTTGATAAAGCACAGCAGAGCTTAAAACAAGGCAGTATTGCAGTGGCAACGGCTGAAGCAAGTACAGTAGATGT
AACTTCAGATGCGCAGCGTTCACGTTTAACTGTAAATTTGACGGATGCAGGTGCATTTACTACACGTGTTGAAGGTAATA
CCTTTATTCTGAAAATTAACTCAGCGAATCCAATGGTAGCAACACCTGTTGCTGCGGCGCGTTCAGAGCAAGGCATTTCA
AATATTGGCTTCCAACGCGGTTCAGGCGGTGAAGGCCAAATTGTGATTGATTTAAAAGGCAGTAATACGCCTGTCGATGT
GAAACAGCAAGGCAGTAAAATTGTGGTGCGCGTTTTGGGGAATAAAATTCCTGCACACTTATCACGCCGTCTCAATGTGA
ATGATTTCGCAACGCCTGTATCGACCATCGATGCACAAAATGATGGTAGTAACGGCCTGATCACAATTCAATCGAATGGC
AGTTTTGAATATATGGCTTATCAGGCAGAAGATAAGCTCACCATTAGTTTAAAGCGTCCTGAAGATAAAAATCCAATGAA
ACCTAAAGCACCAAGTGCTTATACGGGCAAAAAGATTTCTTTAGATTTTCAAGATATTGAAGTGCGACGTGTATTACAGC
TCTTAGCTGATTTTACAGGCATTAACATGGTGGCTTCGGACACAGTACAAGGCAATATTACCTTGCGCCTTAAAGATGTT
CCGTGGGATCAGGCGCTTGATATTATTATGAAAACCAAGAACTTGGATAAGCGACGCAATGGTAATGTGATTTGGATTGC
ACCTGTAGCTGAACTGATCAAATCTGAGGAAGATGAAGCCAAAGCGATTGCGCAAAGTATTAAGCTTTCACCAATACAAA
CCGATTACATTCAATTGAGTTATGCTAAATCGGCGGATGTACTGAAGTTGTTGGAAGACTCTCGTGATAGCAAAGGAAGT
GAGTCAAATCGTACAGCGGGCTCAGACTCCTTGGCTTTGGAAAGTTTGTTGAGTGCTCGTGGTAGTGCAGTTTCTGACTC
GCGTACCAATACGCTAATCATTAATGATACGCAGCAAAATATTGACAAAATCCGACGTATGATCGACTTACTGGATGTCG
CAGTGAAACAGGTGATGGTCGAAGCACGTATTGTGACAGCGTCAACAGACTTTTCTCGTGAATTGGGGATTAAGTGGAGT
GCGAAGAAAGAGGCGGGAAATAATAGCCCAACTTGGGGTGGGGATTTTCAACCAAGTTTTGATTTAGGTGTGGATTTGGC
GACCTCGACAGCAGGAACATTTAACTTTGGTTTATTGAAAATTTCAGACTATATGTTAAACCTTGAACTAACCGCACTTC
AAGCAGATGGACATGGTGAAGTCTTGTCGGCGCCTAAAGTACTTACTGGCGATAAACAGAAAGCCTATATCTTACGTGGT
ACAGAGATTCCTTATCAAACATGGAGTGCGACGGAAGGCTTAAAAACCGAGTTCCGTGCGGCAAACTTGAAGTTAGAAGT
GACGCCAAGCATTACCCCGGATGGTAAAGTTCAGATGGCACTGATGGTTGAGAAAGACTCGATTGGTGACATTACCAATG
ATGGTATTGCAATTGACACCAGTGAATTACAAACCAATGTGTTGGTTGCGGATGGTGAGACTGTCGTACTGGGTGGTATT
TTTGATGATGTGAAACGTAATGACTATCAAAAAGTGCCATTTTTAGGTGATTTACCCGTAGTTGGTAATTTATTTAAGAA
TAATACCAAAACGACGATGCAAACTGAGTTGTTGATTTTTGTGACACCACGAATTGTTAATGACAGTGTTTCAAGAAATC
ATTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(305-348)

AMIN domain (AMIN)

(55-134)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-457)

Bacterial type II and III secretion system protein (Secretin)

(532-686)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

63.862

100

0.667
  pilQ Acinetobacter baumannii strain A118

63.724

100

0.666
  comQ Acinetobacter baylyi ADP1

62.36

100

0.64
  pilQ Pseudomonas aeruginosa PAK

39.451

99.712

0.393
  pilQ Legionella pneumophila strain Lp02

37.736

99.28

0.375
  pilQ Legionella pneumophila strain ERS1305867

38.018

97.406

0.37

Multiple sequence alignment