Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NLA_RS09025 Genome accession   NC_014752
Coordinates   1866484..1868664 (+) Length   726 a.a.
NCBI ID   WP_013449452.1    Uniprot ID   E4ZF17
Organism   Neisseria lactamica 020-06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1861484..1873664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLA_RS09005 (NLA_17440) pilM 1863523..1864638 (+) 1116 WP_003710234.1 type IV pilus biogenesis protein PilM -
  NLA_RS09010 (NLA_17450) pilN 1864641..1865240 (+) 600 WP_013449450.1 PilN domain-containing protein Machinery gene
  NLA_RS09015 (NLA_17460) - 1865241..1865903 (+) 663 WP_003710238.1 type 4a pilus biogenesis protein PilO -
  NLA_RS09020 (NLA_17470) pilP 1865921..1866466 (+) 546 WP_013449451.1 pilus assembly protein PilP Machinery gene
  NLA_RS09025 (NLA_17480) pilQ 1866484..1868664 (+) 2181 WP_013449452.1 type IV pilus secretin PilQ Machinery gene
  NLA_RS09035 (NLA_17490) - 1869847..1870359 (+) 513 WP_013449453.1 shikimate kinase -
  NLA_RS09040 (NLA_17500) aroB 1870445..1871524 (+) 1080 WP_013449454.1 3-dehydroquinate synthase -
  NLA_RS09045 (NLA_17510) - 1872054..1872896 (+) 843 WP_003711015.1 class II glutamine amidotransferase -
  NLA_RS09050 (NLA_17520) - 1872926..1873390 (+) 465 WP_013449455.1 ATP cone domain-containing protein -

Sequence

Protein


Download         Length: 726 a.a.        Molecular weight: 78474.40 Da        Isoelectric Point: 9.8535

>NTDB_id=39082 NLA_RS09025 WP_013449452.1 1866484..1868664(+) (pilQ) [Neisseria lactamica 020-06]
MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKVSFDKEVVNPTGFITSSPARIALDFEQTGISMNQQVL
EYADPLLGKISAAQNSSRARLVLNLNKPGQYNTEVRGNEVWILINESDDTTSAPARPAVKAAPAAPAKQQAAAPFTESVV
SVSAPFSPATQQTAAVQAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQRDHIIVTLKNHTLPTALQRSLDVADFKTP
VQKVTLKRLNNDTQLIITTTGNWELINKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFKGRKISLDFQDVEVRTILQILA
KESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQN
FQLKYKNVEEFRSILRLDNADTTGNRNTLISGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEA
ADGFSRDLGVKFGATGKKKLKDADRAFGWGVNSGFEGGEGGKWSAQTSINLPVAAAANSISLVRAISSGALNLELSASES
LSKTKTLANPRVLTQNRKEAKIESGTEIPFQDTSSSGGSSSTTTTFKKAVLGLTVTPNITPDGQIIMTVKINKDTPVDCT
VKDETTKCIQTKNLNTQAMVENGGTLIVGGIYEEENGNTLTKIPLLGDIPVIGNLFKTRGKSTNRRELLIFITPRIMDTA
GNNLRY

Nucleotide


Download         Length: 2181 bp        

>NTDB_id=39082 NLA_RS09025 WP_013449452.1 1866484..1868664(+) (pilQ) [Neisseria lactamica 020-06]
ATGAATACCAAACTGACAAAAATCATTTCCGGCCTGTTTGTCGCAACCGCCGCCTTTCAGACGGCATCGGCAGGAAACAT
TACAGACATCAAAGTTTCCTCCCTGCCCAACAAACAGAAAATCGTCAAAGTCAGCTTTGACAAAGAGGTTGTCAACCCGA
CCGGCTTCATCACTTCGTCGCCCGCGCGCATCGCCTTGGACTTTGAACAAACCGGCATTTCCATGAATCAACAGGTACTC
GAGTATGCCGACCCTCTGTTGGGCAAAATCAGTGCCGCACAGAACAGCAGCCGTGCGCGTCTGGTTCTGAATCTGAACAA
ACCGGGCCAATACAATACCGAAGTACGCGGAAATGAAGTTTGGATTCTCATCAACGAATCGGACGATACCACATCCGCCC
CCGCCCGCCCCGCCGTAAAAGCCGCGCCTGCCGCACCGGCAAAACAACAGGCTGCCGCACCGTTTACCGAGTCCGTAGTA
TCCGTATCCGCCCCGTTCAGCCCCGCAACACAACAAACGGCGGCTGTACAGGCAAAACAAACCAATATCGATTTCCGCAA
AGACGGCAAAAATGCCGGCATTATCGAATTGGCGGCATTGGGCTTTGCCGGGCAGCCCGACATCAGCCAACAGCGCGACC
ACATCATCGTTACGCTGAAAAACCATACCCTGCCGACCGCGCTCCAACGCAGTTTGGATGTGGCAGACTTCAAAACACCG
GTTCAAAAGGTTACGCTGAAACGCCTCAATAACGACACCCAGCTGATTATCACGACAACCGGCAACTGGGAACTCATCAA
CAAATCCGCCGCACCCGGATACTTTACCTTCCAAGTCCTGCCGAAAAAACAAAACCTCGAGTCAGGCGGCGTGAACAATG
CGCCCAAAACCTTCAAAGGCCGGAAAATCTCCCTTGACTTCCAAGATGTCGAAGTCCGCACCATCCTGCAGATTTTGGCC
AAAGAATCCGGAATGAACATTGTTGCCAGCGACTCCGTCAACGGCAAAATGACCCTCTCCCTCAAAGATGTGCCTTGGGA
TCAGGCTTTGGATTTGGTTATGCAGGCGCGCAACCTCGATATGCGCCAGCAAGGAAACATCGTCAACATCGCGCCCCGCG
ACGAGCTGCTTGCCAAAGACAAAGCCTTCTTACAGGCAGAAAAAGACATCGCCGATTTGGGCGCGCTGTATTCCCAAAAC
TTCCAGTTGAAATACAAAAATGTGGAAGAATTCCGCAGCATCCTGCGTTTGGACAATGCCGACACGACCGGAAACCGCAA
CACGCTTATCAGCGGCAGGGGCAGCGTGCTGATCGATCCCGCCACCAATACCCTGATTGTTACCGATACCCGCAGCGTCA
TCGAAAAATTCCGCAAACTGATCGACGAATTGGACGTGCCCGCGCAACAAGTAATGATTGAGGCGCGCATCGTCGAAGCG
GCAGACGGCTTCTCGCGCGATTTGGGCGTTAAGTTCGGCGCGACAGGCAAGAAAAAACTGAAAGATGCTGACCGCGCATT
CGGCTGGGGCGTGAACTCGGGTTTTGAAGGCGGCGAAGGCGGCAAATGGTCTGCCCAAACCAGCATCAACCTGCCGGTTG
CCGCTGCCGCAAACAGCATTTCGCTGGTGCGCGCGATTTCCTCCGGCGCGTTGAATTTGGAATTGTCCGCATCCGAGTCG
CTTTCAAAAACCAAAACGCTTGCCAATCCGCGCGTGCTGACCCAAAACCGCAAAGAGGCCAAAATCGAATCCGGTACGGA
AATCCCCTTCCAAGACACTTCAAGTTCGGGCGGCAGCAGCAGCACAACCACAACCTTCAAAAAAGCCGTCTTGGGGCTGA
CCGTTACGCCGAACATCACGCCCGACGGCCAAATCATCATGACCGTCAAAATCAATAAAGACACGCCTGTCGACTGTACC
GTGAAAGATGAAACGACCAAGTGTATCCAAACCAAAAACCTGAATACGCAGGCTATGGTTGAAAACGGCGGTACCTTGAT
TGTCGGCGGTATTTATGAAGAAGAGAACGGCAATACGCTGACCAAAATCCCCCTGTTGGGCGACATCCCCGTTATCGGCA
ACCTCTTTAAAACACGAGGGAAAAGCACCAACCGCCGCGAACTGCTGATTTTCATTACCCCGAGGATTATGGATACGGCC
GGCAACAACCTGCGCTATTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(558-718)

Bacterial type II/III secretion system short domain (Secretin_N)

(399-470)

Secretin and TonB N terminus short domain (STN)

(326-371)

AMIN domain (AMIN)

(43-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4ZF17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

92.287

100

0.923
  pilQ Neisseria meningitidis MC58

85.084

100

0.904
  pilQ Neisseria meningitidis M1080

85.714

100

0.901

Multiple sequence alignment