Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   HHJ44_RS15375 Genome accession   NZ_CP053231
Coordinates   3172157..3174238 (+) Length   693 a.a.
NCBI ID   WP_000678408.1    Uniprot ID   B7MFL3
Organism   Escherichia coli strain SCU-306     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3167157..3179238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ44_RS15355 (HHJ44_15350) gmk 3168375..3168998 (+) 624 WP_001295237.1 guanylate kinase -
  HHJ44_RS15360 (HHJ44_15355) rpoZ 3169053..3169328 (+) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  HHJ44_RS15365 (HHJ44_15360) spoT 3169347..3171455 (+) 2109 WP_000280473.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  HHJ44_RS15370 (HHJ44_15365) trmH 3171462..3172151 (+) 690 WP_001070179.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  HHJ44_RS15375 (HHJ44_15370) recG 3172157..3174238 (+) 2082 WP_000678408.1 ATP-dependent DNA helicase RecG Machinery gene
  HHJ44_RS15380 (HHJ44_15375) gltS 3174272..3175477 (-) 1206 WP_128542935.1 sodium/glutamate symporter -
  HHJ44_RS15385 (HHJ44_15380) xanP 3175757..3177148 (+) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  HHJ44_RS15390 (HHJ44_15385) yicH 3177269..3178978 (+) 1710 WP_000668610.1 AsmA family protein -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76480.45 Da        Isoelectric Point: 7.3384

>NTDB_id=390763 HHJ44_RS15375 WP_000678408.1 3172157..3174238(+) (recG) [Escherichia coli strain SCU-306]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFNAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACM
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=390763 HHJ44_RS15375 WP_000678408.1 3172157..3174238(+) (recG) [Escherichia coli strain SCU-306]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCGCTCAGTTCCCTAACGGGCGTTGGCGCAGCGCTTAGTAACAAGCTGGCGAA
AATCAACCTGCATACCGTACAGGATTTGCTCTTACACCTTCCTCTGCGCTACGAAGATCGCACACATCTCTACCCCATCG
GTGAACTACTGCCGGGTGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATTCTCACTATGCGCTTTTTCAACTTCAATGCGGCAATGAAAAATAGCCT
GGCGACAGGCCGCCGCGTGCTGGCCTATGGCGAAGCAAAGCGCGGCAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTACAGGGCGATCTCAGCACGCCAGAGTTACAGGAAACGCTCACGCCGGTTTACCCAACTACGGAAGGCGTAAAGCAG
GCTACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACATGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCGCCGCCGACGCTACAGCTTAGCGATCTCG
AAACCGGGCAGCATCCGGCACAACGCCGGCTGATTCTGGAAGAGTTGCTGGCGCACAACCTCAGCATGTTGGCGTTACGT
GCCGGTGCACAACGTTTTCATGCTCAGCCGCTGAGCGCCAATGACGCGCTGAAAAATAAACTCCTCGCCGCCTTACCGTT
CAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGG
TGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCTGCCCTCGCCGCGTTGCGTGCGGTTGCCCACGGCAAACAGGTG
GCCCTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTTCGTAACTGGTTTGCACCGCTCGGTATCGA
AGTAGGCTGGCTCGCCGGAAAACAGAAAGGTAAAGCACGACTGGCTCAGCAAGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTTGGTACGCACGCCATTTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGTTTTGGCGTACATCAGCGTCTGGCGTTATGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCCATCCCCCGCACGCTGGCGATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCACCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGATATCATTGACCGCGTGCGCCACGCCTGTATG
ACTGAAGGGCGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTACTGGAAGCGCAGGCGGCGGAAGCGAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAATGTTGGCCTGGTACACGGACGGATGAAACCCGCCGAGAAACAGG
CGGTGATGGCGTCGTTTAAACAAGGTGAACTACACCTGCTGGTTGCCACTACTGTCATTGAAGTCGGTGTTGATGTACCC
AATGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGTTACGTGGCCGCGTGGGTCG
TGGCGCGGTGGCCTCTCACTGCGTGCTGCTCTACAAAACGCCGCTCTCCAAAACCGCGCAAATCCGCCTGCAGGTACTAC
GCGACAGCAACGATGGTTTTGTGATTGCACAGAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGTACACGTCAG
ACGGGGAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCGCGACA
TATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCAGAAACAGAACGTTACTCGAATGCGT
AA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(273-433)

Helicase conserved C-terminal domain (Helicase_C)

(481-588)

RecG wedge domain (RecG_wedge)

(15-171)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7MFL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.232

96.97

0.506
  recG/mmsA Streptococcus pneumoniae R6

39.074

99.711

0.39
  recG/mmsA Streptococcus pneumoniae R36A

39.074

99.711

0.39
  recG Bacillus subtilis subsp. subtilis str. 168

39.058

94.949

0.371

Multiple sequence alignment