Detailed information NTG0390253

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FSC11_RS07005
• Genome accession: NZ_CP044483
• Coordinates: 1435374..1436579 (+)
• Length: 401 a.a.
• NCBI ID: WP_048881092.1
• Uniprot ID: A0A0J8SWW2
• Organism: Acinetobacter schindleri strain HZE30-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1430374..1441579

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FSC11_RS06990 (FSC11_06995)	-	1431438..1431968 (+)	531	WP_163165926.1	hypothetical protein	-
FSC11_RS06995 (FSC11_07000)	-	1432490..1433539 (+)	1050	WP_163165928.1	3-deoxy-7-phosphoheptulonate synthase	-
FSC11_RS07000 (FSC11_07005)	pilU	1433784..1434962 (+)	1179	WP_163165930.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FSC11_RS07005 (FSC11_07010)	pilU	1435374..1436579 (+)	1206	WP_048881092.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FSC11_RS07010 (FSC11_07015)	-	1436637..1437815 (-)	1179	WP_163165932.1	MFS transporter	-
FSC11_RS07015 (FSC11_07020)	gcvH	1438045..1438419 (-)	375	WP_004809515.1	glycine cleavage system protein GcvH	-
FSC11_RS07020 (FSC11_07025)	-	1438492..1439520 (-)	1029	WP_163165934.1	AraC family transcriptional regulator	-
FSC11_RS07025 (FSC11_07030)	-	1439673..1440122 (-)	450	WP_004895014.1	MaoC family dehydratase	-

Sequence

Protein

• Length: 401 a.a.
• Molecular weight: 45193.55 Da
• Isoelectric Point: 6.3946

>NTDB_id=390253 FSC11_RS07005 WP_048881092.1 1435374..1436579(+) (pilU) [Acinetobacter schindleri strain HZE30-1]
MFTAELLEEARKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQELTPEKTKLFAYALMNEKQRKEYEAELEC
NFAITVPGVSRFRVNVFQQQLNVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDSHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIVSQRLVRTEDGKGRRAAVEIMLNTPLVSDLILKGDFHELKAIMGKS
RELGMQTFDQALFDLYNEGAISYEEALRNADSTNELRLQIKLKSTRANPAMNNTATAFSMVEEKKPDNETDEDASSTEQS
S

Nucleotide

• Length: 1206 bp

>NTDB_id=390253 FSC11_RS07005 WP_048881092.1 1435374..1436579(+) (pilU) [Acinetobacter schindleri strain HZE30-1]
ATGTTCACGGCAGAGTTATTAGAAGAAGCACGTAAGTTTATGCATCATATGCTGACCAAGGTCGTTGAATATGGTGGTTC
AGACCTGTTCATTACCGCAGACTTTCCTCCGAGTATCAAGCATCAGGGATTAATGAAACCTTTTGGGCAACAGGAACTTA
CCCCAGAAAAAACCAAACTGTTTGCCTATGCACTGATGAACGAAAAGCAGCGTAAAGAATATGAAGCAGAACTGGAATGT
AACTTTGCCATTACTGTACCGGGCGTTTCACGTTTCCGTGTCAACGTGTTCCAGCAGCAGCTCAATGTCGGTATGGTGAT
CCGGACCATCACTGCAGAAATTCCGAATTTCCAGAAGTTAAAATTGCCAGAGTCACTCAAGCATGTGATTATGGAAAAGC
GCGGTCTGGTACTCGTCGTGGGCGGTACAGGTTCAGGTAAGTCGACTTCACTTGCTGCGATGATTGATTATCGTAATGAA
AACTCAGCAGGCCATATTATCACCGTAGAAGACCCGGTGGAATATGTACATAAACATAAGAAGTCAATGATTACCCATCG
CGAGGTCGGTGTAGATAGCCATTCCTGGCATAATGCCTTAAAAAATACCTTGCGTCAGGCACCCGATGTGATTCTGATTG
GTGAAATCCGGGATACGGAAACCATGGAACATGCGATTGCCTTTGCCGAAACAGGGCATCTCTGTCTGGGGACGCTGCAT
TCCAACAATGCCAACCAGACGCTCGACCGGATTATTAACTTCTTCCCGGAGGAACGCCGTAATCAGTTATTGATGGATCT
GTCTTCCAATATGAAAGCGATTGTCTCTCAGCGTTTGGTCCGTACTGAAGATGGCAAGGGGCGTCGTGCCGCAGTCGAAA
TTATGCTGAATACACCATTGGTATCGGATCTGATTCTGAAAGGTGATTTCCATGAATTAAAAGCAATTATGGGTAAATCA
CGCGAACTTGGTATGCAGACTTTTGATCAGGCATTATTTGATCTTTATAACGAAGGTGCAATTTCTTATGAAGAAGCATT
GCGTAATGCTGATTCGACCAATGAACTTCGTCTGCAAATCAAACTGAAAAGTACGCGTGCTAACCCAGCCATGAATAATA
CAGCCACCGCATTTAGTATGGTGGAAGAGAAAAAGCCGGATAATGAAACGGATGAAGATGCCAGTAGCACAGAACAAAGC
TCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (117-282)

3D structure

• AlphaFold DB: A0A0J8SWW2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.207	86.783	0.531
pilU	Acinetobacter baylyi ADP1	54.19	89.277	0.484
pilU	Vibrio cholerae strain A1552	50.986	88.529	0.451