Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   F5K02_RS13170 Genome accession   NZ_CP044132
Coordinates   2684815..2685885 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain ZKY01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2679815..2690885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F5K02_RS13120 (F5K02_13190) - 2679875..2680459 (-) 585 WP_015240205.1 signal peptidase I -
  F5K02_RS13125 (F5K02_13195) tapA 2680431..2681102 (-) 672 WP_015417813.1 amyloid fiber anchoring/assembly protein TapA -
  F5K02_RS13130 (F5K02_13200) - 2681361..2681690 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  F5K02_RS13135 (F5K02_13205) - 2681730..2681909 (-) 180 WP_003153093.1 YqzE family protein -
  F5K02_RS13140 (F5K02_13210) comGG 2681966..2682343 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  F5K02_RS13145 (F5K02_13215) comGF 2682344..2682844 (-) 501 WP_254922226.1 competence type IV pilus minor pilin ComGF -
  F5K02_RS13150 (F5K02_13220) comGE 2682753..2683067 (-) 315 WP_094032244.1 competence type IV pilus minor pilin ComGE -
  F5K02_RS13155 (F5K02_13225) comGD 2683051..2683488 (-) 438 WP_094032243.1 competence type IV pilus minor pilin ComGD Machinery gene
  F5K02_RS13160 (F5K02_13230) comGC 2683478..2683786 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  F5K02_RS13165 (F5K02_13235) comGB 2683791..2684828 (-) 1038 WP_015417819.1 competence type IV pilus assembly protein ComGB Machinery gene
  F5K02_RS13170 (F5K02_13240) comGA 2684815..2685885 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  F5K02_RS13175 (F5K02_13245) - 2686077..2687027 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  F5K02_RS13180 (F5K02_13250) - 2687173..2688474 (+) 1302 WP_241457553.1 hemolysin family protein -
  F5K02_RS13185 (F5K02_13255) - 2688512..2689342 (-) 831 WP_015417821.1 STAS domain-containing protein -
  F5K02_RS13195 (F5K02_13265) - 2689549..2689929 (-) 381 WP_064115382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  F5K02_RS13200 (F5K02_13270) - 2690166..2690411 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=388106 F5K02_RS13170 WP_003153083.1 2684815..2685885(-) (comGA) [Bacillus amyloliquefaciens strain ZKY01]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=388106 F5K02_RS13170 WP_003153083.1 2684815..2685885(-) (comGA) [Bacillus amyloliquefaciens strain ZKY01]
GTGAAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment