Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HHJ37_RS01950 Genome accession   NZ_CP051706
Coordinates   423814..425295 (-) Length   493 a.a.
NCBI ID   WP_001219885.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-124     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418814..430295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ37_RS01920 (HHJ37_01920) gspK 419727..420710 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  HHJ37_RS01925 (HHJ37_01925) gspJ 420703..421290 (-) 588 WP_001525809.1 type II secretion system minor pseudopilin GspJ -
  HHJ37_RS01930 (HHJ37_01930) gspI 421283..421660 (-) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  HHJ37_RS01935 (HHJ37_01935) gspH 421657..422166 (-) 510 WP_001525808.1 type II secretion system minor pseudopilin GspH -
  HHJ37_RS01940 (HHJ37_01940) gspG 422174..422611 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  HHJ37_RS01945 (HHJ37_01945) gspF 422621..423817 (-) 1197 WP_001109563.1 type II secretion system inner membrane protein GspF -
  HHJ37_RS01950 (HHJ37_01950) pilB 423814..425295 (-) 1482 WP_001219885.1 type II secretion system ATPase GspE Machinery gene
  HHJ37_RS01955 (HHJ37_01955) gspD 425305..427257 (-) 1953 WP_001525807.1 type II secretion system secretin GspD -
  HHJ37_RS01960 (HHJ37_01960) gspC 427241..428056 (-) 816 WP_001525806.1 type II secretion system protein GspC -
  HHJ37_RS01965 (HHJ37_01965) gspA 428236..429705 (+) 1470 WP_001525805.1 type II secretion system protein GspA -
  HHJ37_RS01970 (HHJ37_01970) gspB 429707..430126 (+) 420 WP_000461443.1 putative general secretion pathway protein GspB -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54705.71 Da        Isoelectric Point: 6.2412

>NTDB_id=386963 HHJ37_RS01950 WP_001219885.1 423814..425295(-) (pilB) [Escherichia coli strain SCU-124]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNIPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
HHQLAVTTIGTPVGCPYCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
VMRVASVTLESEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=386963 HHJ37_RS01950 WP_001219885.1 423814..425295(-) (pilB) [Escherichia coli strain SCU-124]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACGGTTACTCGATATTCAACGTCAAGTTGGTCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGACTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCTATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCCAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGGATCAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCATCCATCTATGGTGAACGCGCTGTGCTCCGTCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTTAA
TATCCCTGGCCGTAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAAATTGCGGTTCAGGCCTCGCTCACAGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTCACCCGGCTACGCGACATGGGCGTCGAATCATTCCTGCTTTCATCTTCACTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGTCTGTGTCCGCAATGCCGACAATTCACGCCTGTATCGCCGCAACAAGCGCAGATGTTTAAA
CATCATCAGCTCGCGGTGACGACAATTGGCACTCCCGTAGGTTGCCCTTATTGCCATCAATCCGGTTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTGCGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATACGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGTCACGCTGGAGAGCGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.697

89.047

0.416
  pilB Vibrio cholerae strain A1552

48.201

84.584

0.408
  pilB Acinetobacter baumannii D1279779

45.455

89.249

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

46.588

86.207

0.402
  pilB Legionella pneumophila strain ERS1305867

41.164

97.566

0.402
  pilB/pilB1 Synechocystis sp. PCC 6803

41.176

96.552

0.398
  pilB Acinetobacter baylyi ADP1

48.338

79.31

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.293

79.716

0.361