Detailed information NTG0386788

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: F3P16_RS14380
• Genome accession: NZ_CP043953
• Coordinates: 3061549..3062667 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain K09-14
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3056549..3067667

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F3P16_RS14370 (F3P16_14370)	-	3059665..3060357 (-)	693	WP_032034779.1	YggS family pyridoxal phosphate-dependent enzyme	-
F3P16_RS14375 (F3P16_14375)	pilT	3060484..3061521 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
F3P16_RS14380 (F3P16_14380)	pilU	3061549..3062667 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
F3P16_RS14385 (F3P16_14385)	fur	3062737..3063174 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
F3P16_RS14390 (F3P16_14390)	-	3063286..3063684 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
F3P16_RS14395 (F3P16_14395)	-	3063739..3064062 (-)	324	WP_000442590.1	RnfH family protein	-
F3P16_RS14400 (F3P16_14400)	-	3064062..3065129 (-)	1068	WP_004748097.1	hypothetical protein	-
F3P16_RS14410 (F3P16_14410)	-	3065282..3065737 (-)	456	WP_000782976.1	bacteriohemerythrin	-
F3P16_RS14415 (F3P16_14415)	-	3065990..3066838 (-)	849	WP_025469635.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=386788 F3P16_RS14380 WP_000347036.1 3061549..3062667(+) (pilU) [Acinetobacter baumannii strain K09-14]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=386788 F3P16_RS14380 WP_000347036.1 3061549..3062667(+) (pilU) [Acinetobacter baumannii strain K09-14]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAATAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392