Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HHJ46_RS03860 Genome accession   NZ_CP051694
Coordinates   808739..810232 (+) Length   497 a.a.
NCBI ID   WP_000249331.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-313     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 803739..815232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ46_RS03840 (HHJ46_03845) pilD 804387..805196 (+) 810 WP_000895863.1 prepilin peptidase PppA Machinery gene
  HHJ46_RS03845 (HHJ46_03850) gspS2 805262..805672 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  HHJ46_RS03850 (HHJ46_03855) gspC 805690..806649 (+) 960 WP_024192740.1 type II secretion system protein GspC -
  HHJ46_RS03855 (HHJ46_03860) gspD 806679..808739 (+) 2061 WP_000498828.1 type II secretion system secretin GspD -
  HHJ46_RS03860 (HHJ46_03865) pilB/pilB1 808739..810232 (+) 1494 WP_000249331.1 type II secretion system ATPase GspE Machinery gene
  HHJ46_RS03865 (HHJ46_03870) gspF 810232..811455 (+) 1224 WP_001542361.1 type II secretion system inner membrane protein GspF -
  HHJ46_RS03870 (HHJ46_03875) gspG 811472..811927 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  HHJ46_RS03875 (HHJ46_03880) gspH 811931..812494 (+) 564 WP_001542360.1 type II secretion system minor pseudopilin GspH -
  HHJ46_RS03880 (HHJ46_03885) gspI 812491..812862 (+) 372 WP_000820109.1 type II secretion system minor pseudopilin GspI -
  HHJ46_RS03885 (HHJ46_03890) gspJ 812859..813464 (+) 606 WP_001542359.1 type II secretion system minor pseudopilin GspJ -
  HHJ46_RS03890 (HHJ46_03895) gspK 813461..814438 (+) 978 WP_000633203.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55214.35 Da        Isoelectric Point: 6.0089

>NTDB_id=386720 HHJ46_RS03860 WP_000249331.1 808739..810232(+) (pilB/pilB1) [Escherichia coli strain SCU-313]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=386720 HHJ46_RS03860 WP_000249331.1 808739..810232(+) (pilB/pilB1) [Escherichia coli strain SCU-313]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTACAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCTATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAAGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGGCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGCGTCTCCACCATGCCTTCCGCCTGGGGTG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCACGAACTGACC
GCGCAGTTGCGCCAGCTACTGCACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACTGCAGAAATTGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGGGTAGAGCCTTTCCTGC
TCTCTTCCAGTCTGACTGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGCCCCGACTGCCGCCAGCCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

45

80.483

0.362