Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   F1E05_RS03930 Genome accession   NZ_CP043929
Coordinates   819899..820960 (-) Length   353 a.a.
NCBI ID   WP_150046928.1    Uniprot ID   -
Organism   Methylomonas rhizoryzae strain GJ1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 814899..825960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1E05_RS03910 pilQ 815853..818153 (-) 2301 WP_150046921.1 type IV pilus secretin family protein -
  F1E05_RS03915 - 818167..818715 (-) 549 WP_150046923.1 pilus assembly protein PilP -
  F1E05_RS03920 - 818719..819330 (-) 612 WP_150046925.1 type 4a pilus biogenesis protein PilO -
  F1E05_RS03925 - 819327..819899 (-) 573 WP_150046926.1 PilN domain-containing protein -
  F1E05_RS03930 pilM 819899..820960 (-) 1062 WP_150046928.1 pilus assembly protein PilM Machinery gene
  F1E05_RS03935 - 821276..823648 (+) 2373 WP_150046930.1 penicillin-binding protein 1A -
  F1E05_RS03940 rpmE 823712..823912 (-) 201 WP_150046932.1 50S ribosomal protein L31 -
  F1E05_RS03945 - 824006..825361 (-) 1356 WP_150046934.1 AmpG family muropeptide MFS transporter -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38220.80 Da        Isoelectric Point: 4.8018

>NTDB_id=386588 F1E05_RS03930 WP_150046928.1 819899..820960(-) (pilM) [Methylomonas rhizoryzae strain GJ1]
MSWFNRSQAALLGIDISTAAVKLLELSRTGARYKVESYAVAPLPQDAVIDKNITNVEVIGEAIKAAARQSGTRAKRACVA
VAGSSVMTKIITMPASLTEADMEEQIMVEADQYIPYSLDEVNLDFEVQGPSKSNPEMLDVLLAASRRENIEDRVAALNYA
GLKAAIVDVEAFAMENAFTLLVDQLPENMANKTVAIADVGATLTSLNILHNGKTIYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKRHGGLPDNYIPDVLEPFKKAMLQQIARSLQFFVSSSANRGIDALILAGGCASIPGLEKLVERDLGLSSYIANPF
INMALSNRVKPQNLSNDTPAMMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=386588 F1E05_RS03930 WP_150046928.1 819899..820960(-) (pilM) [Methylomonas rhizoryzae strain GJ1]
ATGAGCTGGTTTAACAGGAGTCAGGCCGCTTTACTCGGCATAGACATTAGCACTGCAGCCGTCAAACTGTTGGAATTGAG
TCGGACGGGCGCGCGCTATAAAGTCGAGAGTTACGCTGTCGCTCCTCTGCCACAAGACGCCGTGATCGACAAAAACATTA
CCAATGTCGAAGTAATAGGCGAAGCGATCAAAGCTGCGGCCAGACAATCCGGCACCCGTGCGAAACGCGCTTGCGTCGCG
GTAGCTGGTTCTTCGGTGATGACCAAAATCATCACCATGCCGGCCTCCCTGACCGAGGCCGACATGGAAGAACAAATCAT
GGTCGAAGCCGATCAATACATTCCTTATTCGCTGGACGAAGTGAATTTGGATTTCGAAGTACAAGGCCCGTCCAAAAGTA
ATCCGGAAATGCTCGATGTGCTGCTGGCCGCTTCCAGACGCGAAAACATAGAGGATAGAGTAGCGGCGTTGAATTACGCC
GGATTGAAAGCTGCCATCGTAGACGTGGAAGCGTTTGCAATGGAAAACGCGTTTACCTTGTTGGTCGATCAATTACCCGA
AAACATGGCCAATAAAACCGTTGCGATTGCCGACGTCGGCGCAACCCTGACTTCGCTGAACATCTTGCACAACGGCAAAA
CCATTTACACGCGGGAACAGGGCTTCGGCGGCAAACAACTAACCGAAGAAATCCAACGCCGTTACGGCTTGTCTTACGAA
GAAGCCGGTTTGGCGAAACGGCACGGCGGCTTGCCGGACAATTACATTCCCGACGTGTTGGAGCCTTTTAAAAAGGCCAT
GTTGCAACAAATCGCCCGCTCGCTGCAGTTCTTCGTATCCTCCAGCGCCAACCGCGGCATAGACGCTTTAATTTTAGCCG
GTGGTTGCGCATCGATTCCGGGCCTGGAAAAACTGGTCGAACGGGACTTGGGTTTGTCGTCGTACATCGCCAACCCGTTT
ATCAACATGGCGTTATCCAACAGAGTCAAGCCGCAAAACTTGAGTAACGATACCCCGGCGATGATGATCGCTTGCGGTCT
GGCCTTGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.142

100

0.501
  comM Acinetobacter nosocomialis M2

50.142

100

0.501
  comM Acinetobacter baylyi ADP1

47.875

100

0.479
  pilM Legionella pneumophila strain ERS1305867

41.36

100

0.414

Multiple sequence alignment