Detailed information NTG0386531

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AT571_RS11725
• Genome accession: NZ_CP043910
• Coordinates: 2453103..2454221 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain AB043
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2448103..2459221

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AT571_RS11690 (AT571_11690)	-	2448932..2449780 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
AT571_RS11695 (AT571_11695)	-	2450033..2450488 (+)	456	WP_000782976.1	bacteriohemerythrin	-
AT571_RS11705 (AT571_11705)	-	2450641..2451708 (+)	1068	WP_004748097.1	hypothetical protein	-
AT571_RS11710 (AT571_11710)	-	2451708..2452031 (+)	324	WP_000442590.1	RnfH family protein	-
AT571_RS11715 (AT571_11715)	-	2452086..2452484 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AT571_RS11720 (AT571_11720)	fur	2452596..2453033 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AT571_RS11725 (AT571_11725)	pilU	2453103..2454221 (-)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AT571_RS11730 (AT571_11730)	pilT	2454249..2455286 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AT571_RS11735 (AT571_11735)	-	2455413..2456105 (+)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=386531 AT571_RS11725 WP_000347034.1 2453103..2454221(-) (pilU) [Acinetobacter baumannii strain AB043]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=386531 AT571_RS11725 WP_000347034.1 2453103..2454221(-) (pilU) [Acinetobacter baumannii strain AB043]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39