Detailed information NTG0038585

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NMBB_RS00265
• Genome accession: NC_017505
• Coordinates: 50639..51865 (-)
• Length: 408 a.a.
• NCBI ID: WP_002218483.1
• Uniprot ID: Q9K1N4
• Organism: Neisseria meningitidis alpha710
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 45639..56865

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NMBB_RS00260 (NMBB_0054)	yccS	48203..50353 (-)	2151	WP_002244729.1	YccS family putative transporter	-
NMBB_RS00265 (NMBB_0055)	pilU	50639..51865 (-)	1227	WP_002218483.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NMBB_RS00270 (NMBB_0056)	pilT	52028..53071 (-)	1044	WP_002218487.1	type IV pilus twitching motility protein PilT	Machinery gene
NMBB_RS00275 (NMBB_0057)	-	53191..53877 (+)	687	WP_002231428.1	YggS family pyridoxal phosphate-dependent enzyme	-
NMBB_RS00280 (NMBB_0058)	-	53877..54285 (+)	409	Protein_51	RNA-binding protein	-
NMBB_RS00285 (NMBB_0059)	proC	54316..55125 (+)	810	WP_226879339.1	pyrroline-5-carboxylate reductase	-
NMBB_RS00290 (NMBB_0060)	dksA	55272..55688 (+)	417	WP_002231430.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45674.28 Da
• Isoelectric Point: 6.9640

>NTDB_id=38585 NMBB_RS00265 WP_002218483.1 50639..51865(-) (pilU) [Neisseria meningitidis alpha710]
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=38585 NMBB_RS00265 WP_002218483.1 50639..51865(-) (pilU) [Neisseria meningitidis alpha710]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAATGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCGCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAGTCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTAGGCCTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCAGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCCGACGTGGTCTGCATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATCGACATCGCCC
TCAACCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAACAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: Q9K1N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.598	88.48	0.395
pilU	Pseudomonas stutzeri DSM 10701	44.663	87.255	0.39
pilU	Vibrio cholerae strain A1552	46.429	82.353	0.382