Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   F1537_RS04540 Genome accession   NZ_CP043770
Coordinates   857418..858635 (+) Length   405 a.a.
NCBI ID   WP_013525532.1    Uniprot ID   A0AAV2U474
Organism   Haemophilus influenzae biotype aegyptius strain HE7/F1946     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 852418..863635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1537_RS04520 (F1537_04530) corC 853384..854283 (-) 900 WP_013525534.1 CNNM family magnesium/cobalt transport protein CorC -
  F1537_RS04525 (F1537_04535) ampD 854906..855466 (-) 561 WP_006996183.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  F1537_RS04530 (F1537_04540) pilA 855581..856030 (+) 450 WP_006996184.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  F1537_RS04535 (F1537_04545) pilB 856027..857421 (+) 1395 WP_013525533.1 GspE/PulE family protein Machinery gene
  F1537_RS04540 (F1537_04550) pilC 857418..858635 (+) 1218 WP_013525532.1 type II secretion system F family protein Machinery gene
  F1537_RS04545 (F1537_04555) pilD 858632..859324 (+) 693 WP_013525531.1 A24 family peptidase Machinery gene
  F1537_RS04550 (F1537_04560) rho 859379..860641 (-) 1263 WP_005666690.1 transcription termination factor Rho -
  F1537_RS04555 (F1537_04565) metJ 860889..861206 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  F1537_RS04560 (F1537_04570) cueR 861220..861606 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  F1537_RS04565 (F1537_04575) - 861683..861889 (+) 207 WP_013525530.1 heavy-metal-associated domain-containing protein -
  F1537_RS04570 (F1537_04580) - 861964..862170 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  F1537_RS04580 (F1537_04590) - 862256..862462 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 46062.22 Da        Isoelectric Point: 9.7233

>NTDB_id=385769 F1537_RS04540 WP_013525532.1 857418..858635(+) (pilC) [Haemophilus influenzae biotype aegyptius strain HE7/F1946]
MTKKLFYYQGSNALNQKQKGSIIADTKQQAHFQLISRGLTHIKLQQNWQFGAKPKNSEISELLNQLATLLQSAIPLKNSL
QILQQNCTQIMLNEWLERLLQSIESGLAFSQAIEQQGKYLTQQEIQLIQVGEMTGKLAVVCKKIATHRSQSLALQRKLQK
IMLYPSMVLGISLLLTLALLLFIVPQFAEMYSGNNAELPTITAILLSISNFLKQNIGILLFFALSFVLFYYFYLKRQTWF
HQKKNQLISITPIFGTIQKLSRLVNFSHSLQIMLQAGVPLNQALDSFLPRTQTWQTKTLVNDIILDKEVRSILQWVSQGY
AFSDSVSSDLFPMEAQQMLQIGEQSGKLALMLEHIADNYQEKLNHQIDLLSQMLEPLMMVIIGSLIGIIMMGMYLPIFNM
GSVIQ

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=385769 F1537_RS04540 WP_013525532.1 857418..858635(+) (pilC) [Haemophilus influenzae biotype aegyptius strain HE7/F1946]
ATGACTAAAAAACTCTTTTATTATCAAGGTAGTAACGCATTAAATCAGAAACAAAAAGGCTCAATTATTGCGGATACGAA
ACAACAAGCGCACTTTCAGTTAATAAGCCGCGGGCTTACTCACATCAAATTACAACAAAACTGGCAATTTGGGGCAAAAC
CCAAAAATTCAGAAATCAGTGAATTACTCAATCAATTAGCGACATTGCTACAGTCCGCAATTCCGTTAAAAAACAGCCTG
CAAATTTTGCAACAAAATTGTACTCAAATTATGCTCAATGAATGGCTTGAACGGCTGCTTCAATCCATTGAATCTGGCTT
AGCATTCTCACAAGCCATTGAACAACAAGGAAAATATCTCACACAACAAGAAATTCAACTGATTCAAGTGGGAGAAATGA
CAGGAAAACTTGCCGTAGTTTGTAAAAAAATAGCCACGCACCGTAGTCAATCTTTGGCTTTACAACGCAAATTACAGAAA
ATTATGTTATATCCCTCAATGGTATTGGGAATTTCTCTATTATTGACACTCGCATTACTGCTTTTTATCGTGCCTCAATT
TGCTGAAATGTACAGTGGCAACAATGCCGAATTACCAACAATAACTGCAATATTGCTCTCTATATCCAATTTTCTTAAGC
AAAATATTGGCATTTTGCTATTTTTCGCTTTGAGTTTTGTTCTATTTTATTACTTTTATCTAAAACGCCAGACTTGGTTT
CATCAAAAGAAAAATCAACTTATTTCTATCACGCCTATTTTTGGCACAATTCAAAAACTTTCACGTTTAGTGAACTTTAG
TCACAGTTTACAAATTATGTTACAGGCTGGCGTACCGCTTAATCAAGCACTAGACAGTTTTCTCCCTCGCACACAAACGT
GGCAAACCAAGACGCTTGTAAATGACATCATATTAGATAAAGAAGTCCGGTCAATTTTACAATGGGTTTCTCAAGGCTAT
GCGTTTTCTGATAGTGTAAGTAGCGATCTTTTCCCGATGGAAGCACAACAAATGTTACAAATTGGCGAGCAAAGCGGAAA
ACTCGCTTTGATGCTAGAGCATATTGCGGATAATTACCAAGAAAAACTTAATCATCAAATTGACTTACTCTCACAAATGC
TAGAACCATTAATGATGGTAATTATCGGCAGTCTGATTGGAATTATTATGATGGGAATGTATTTACCTATCTTTAATATG
GGCTCTGTTATTCAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(266-396)

Type II secretion system (T2SS), protein F (T2SSF)

(63-185)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Haemophilus influenzae 86-028NP

97.537

100

0.978
  pilC Haemophilus influenzae Rd KW20

97.291

100

0.975
  pilC Glaesserella parasuis strain SC1401

38.5

98.765

0.38

Multiple sequence alignment