Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HHM37_RS10845 Genome accession   NZ_CP051609
Coordinates   2251962..2253443 (+) Length   493 a.a.
NCBI ID   WP_001219886.1    Uniprot ID   -
Organism   Escherichia coli O25b:H4-ST131 strain 2019_APHA     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2246962..2258443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHM37_RS10825 (HHM37_10820) gspB 2247131..2247550 (-) 420 WP_000461439.1 putative general secretion pathway protein GspB -
  HHM37_RS10830 (HHM37_10825) gspA 2247552..2249021 (-) 1470 WP_000107579.1 type II secretion system protein GspA -
  HHM37_RS10835 (HHM37_10830) gspC 2249201..2250016 (+) 816 WP_001142680.1 type II secretion system protein GspC -
  HHM37_RS10840 (HHM37_10835) gspD 2250000..2251952 (+) 1953 WP_001525807.1 type II secretion system secretin GspD -
  HHM37_RS10845 (HHM37_10840) pilB 2251962..2253443 (+) 1482 WP_001219886.1 type II secretion system ATPase GspE Machinery gene
  HHM37_RS10850 (HHM37_10845) gspF 2253440..2254636 (+) 1197 WP_001109564.1 type II secretion system inner membrane protein GspF -
  HHM37_RS10855 (HHM37_10850) gspG 2254646..2255083 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  HHM37_RS10860 (HHM37_10855) gspH 2255091..2255600 (+) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  HHM37_RS10865 (HHM37_10860) gspI 2255597..2255974 (+) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  HHM37_RS10870 (HHM37_10865) gspJ 2255967..2256554 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  HHM37_RS10875 (HHM37_10870) - 2256547..2256672 (+) 126 Protein_2162 type II secretion system protein K -
  HHM37_RS10880 (HHM37_10875) - 2256745..2257914 (+) 1170 Protein_2163 IS3-like element ISEc52 family transposase -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54676.67 Da        Isoelectric Point: 6.3746

>NTDB_id=385628 HHM37_RS10845 WP_001219886.1 2251962..2253443(+) (pilB) [Escherichia coli O25b:H4-ST131 strain 2019_APHA]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNIPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQTVIRGDTSWDE
VMRVASATLENKA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=385628 HHM37_RS10845 WP_001219886.1 2251962..2253443(+) (pilB) [Escherichia coli O25b:H4-ST131 strain 2019_APHA]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACGGTTACTCGATATTCAACGTCAAGTTGGTCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGACTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCTATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCCAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGGATCAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCATCCATCTATGGTGAACGCGCTGTGCTCCGTCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTTAA
TATCCCTGGCCGTAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGACAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAAATTGCGGTTCAGGCCTCGCTCACAGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTCACCCGGCTACGCGACATGGGCGTCGAATCATTCCTGCTTTCATCTTCACTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGTCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCGCCCCAGCAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGTTGCCCTCATTGCCATCAATCCGGTTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTGCGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAACAGTGATACGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCTACGCTGGAGAACAAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.453

88.641

0.412
  pilB Vibrio cholerae strain A1552

47.962

84.584

0.406
  pilB Acinetobacter baumannii D1279779

45.227

89.249

0.404
  pilB Legionella pneumophila strain ERS1305867

40.956

97.566

0.4
  pilB Vibrio parahaemolyticus RIMD 2210633

46.118

86.207

0.398
  pilB/pilB1 Synechocystis sp. PCC 6803

41.202

94.523

0.389
  pilB Acinetobacter baylyi ADP1

48.082

79.31

0.381

Multiple sequence alignment