Detailed information NTG0385497

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HHA37_RS02045
• Genome accession: NZ_CP051547
• Coordinates: 441273..442421 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain AA2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 436273..447421

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HHA37_RS02020 (HHA37_02025)	-	437424..437720 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
HHA37_RS02025 (HHA37_02030)	-	437717..438310 (-)	594	WP_003084544.1	YggT family protein	-
HHA37_RS02030 (HHA37_02035)	proC	438321..439142 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HHA37_RS02035 (HHA37_02040)	-	439154..439846 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HHA37_RS02040 (HHA37_02045)	pilT	440061..441095 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HHA37_RS02045 (HHA37_02050)	pilU	441273..442421 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HHA37_RS02050 (HHA37_02055)	czcD	442429..443328 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HHA37_RS02055 (HHA37_02060)	-	443483..443887 (+)	405	WP_003084560.1	NINE protein	-
HHA37_RS02060 (HHA37_02065)	-	444130..445503 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
HHA37_RS02065 (HHA37_02070)	-	445500..446684 (+)	1185	WP_044061315.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=385497 HHA37_RS02045 WP_003100959.1 441273..442421(+) (pilU) [Pseudomonas aeruginosa strain AA2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=385497 HHA37_RS02045 WP_003100959.1 441273..442421(+) (pilU) [Pseudomonas aeruginosa strain AA2]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATTACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGATTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382