Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F0P95_RS16155 Genome accession   NZ_CP043567
Coordinates   3543442..3545172 (-) Length   576 a.a.
NCBI ID   WP_182335078.1    Uniprot ID   -
Organism   Stenotrophomonas acidaminiphila strain T0-18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3538442..3550172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0P95_RS16145 - 3539020..3540942 (-) 1923 WP_182335076.1 sensor histidine kinase -
  F0P95_RS16150 - 3540945..3543137 (-) 2193 WP_182335077.1 EAL domain-containing protein -
  F0P95_RS16155 pilB 3543442..3545172 (-) 1731 WP_182335078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F0P95_RS16160 galE 3545389..3546363 (-) 975 WP_182335079.1 UDP-glucose 4-epimerase GalE -
  F0P95_RS16165 - 3546502..3547359 (+) 858 WP_182335080.1 glycosyltransferase family 2 protein -
  F0P95_RS16170 - 3547416..3548195 (+) 780 WP_182335081.1 class I SAM-dependent methyltransferase -
  F0P95_RS16175 - 3548192..3549133 (+) 942 WP_182335082.1 lysylphosphatidylglycerol synthase domain-containing protein -
  F0P95_RS16180 - 3549130..3549978 (+) 849 WP_182335083.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62443.62 Da        Isoelectric Point: 5.7586

>NTDB_id=385183 F0P95_RS16155 WP_182335078.1 3543442..3545172(-) (pilB) [Stenotrophomonas acidaminiphila strain T0-18]
MNTAVAANLVGITGIARRLVQDGALDETAARNAMAQAATAKVPLAQWLAEKKLVNAAQLAAANAVEFGMPLLDASVFDAN
HNAMKLVSEELLRKHNVLPLFRRGGKLFIGTSNPTQSLDEIKFHTNLVVEPILVDEDQIRRTLDLWVASNESLTGGLGDD
EEGMGDLDVAAGDEDMGGGGDTGIDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRFRIDGLLKTVARAPVK
LRERIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPVQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKKPAHLPDN
ALLAEGFTQAQIDNGIQLYEPVGCEECTEGYKGRTGIYQVMPMTDEIATIVLQGGNALDIAEAAQKIGVNDLRQSALLKA
AQGITSLTEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=385183 F0P95_RS16155 WP_182335078.1 3543442..3545172(-) (pilB) [Stenotrophomonas acidaminiphila strain T0-18]
ATGAATACAGCCGTTGCCGCCAATCTCGTGGGCATCACCGGGATCGCCCGCCGCCTGGTGCAGGACGGTGCGCTGGACGA
GACCGCGGCGCGCAACGCGATGGCGCAGGCGGCGACGGCCAAGGTGCCGCTGGCGCAGTGGCTGGCCGAGAAGAAGCTGG
TCAACGCCGCGCAGCTGGCCGCGGCCAATGCCGTCGAGTTCGGCATGCCGCTGCTGGATGCCTCGGTGTTCGACGCCAAC
CACAACGCGATGAAGCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAGGCGCGGCGGCAAGCT
GTTCATCGGCACCAGCAACCCGACCCAGTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGGCGCACGCTCGACCTGTGGGTGGCGAGCAACGAAAGCCTGACCGGCGGACTGGGCGACGAC
GAGGAGGGCATGGGCGACCTGGACGTCGCCGCCGGTGACGAGGACATGGGGGGCGGCGGCGATACCGGCATCGATGCCAA
GGGCGACGACACCCCGGTGGTCAAGTTCGTCAACAAGGTACTGGTGGACGCGATCCGCCGCGGCGCCTCGGACATCCACT
TCGAGCCCTACGAAGACGACTACCGGGTGCGGTTCCGCATCGACGGCCTGCTCAAGACGGTGGCCAGGGCGCCGGTGAAG
CTGCGCGAGCGCATCGCCGCACGCCTGAAGGTGATGTCGCAGCTGGACATAGCCGAGAAGCGGGTGCCGCAGGACGGCCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACGCTGTTCGGCGAAAAGATCG
TGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTCGGCATCGACAAGCTGGGTTACGAGCCGGTGCAGCAGAAGCTGTTC
CTGGATGCCATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGCCCCACCGGTTCGGGCAAGACCGTGTCGCTGTATAC
CGCGCTGGGCATCCTCAACGACGAGACGCGCAATATCTCCACCGCCGAGGACCCGGTCGAAATCCGCCTGCCGGGCGTGA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGTGACCTGGAGACCGCGGAAATCGCCATCAAGGCGGCGCAGACCGGCCATATGGTGCTGTC
CACCCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCCCCGTACAACATCACCTCCT
CGGTCACCCTGGTCATCGCCCAGCGCCTGGCGCGGCGCCTGTGCTCCAACTGCAAGAAGCCGGCGCACCTGCCGGACAAT
GCGCTGCTGGCCGAGGGTTTCACCCAGGCGCAGATCGACAACGGCATCCAGCTCTACGAGCCGGTCGGCTGCGAGGAGTG
CACCGAGGGTTACAAGGGGCGTACCGGCATCTACCAGGTGATGCCGATGACGGACGAGATCGCCACGATCGTGCTGCAGG
GCGGCAATGCGCTGGACATCGCCGAGGCCGCGCAGAAGATCGGGGTCAACGACCTGCGCCAGTCGGCCCTGCTCAAGGCC
GCGCAGGGCATCACCAGCCTGACCGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-145)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.401

99.653

0.552
  pilB Acinetobacter baylyi ADP1

55.634

98.611

0.549
  pilB Legionella pneumophila strain ERS1305867

53.022

100

0.533
  pilB Vibrio cholerae strain A1552

48.955

99.653

0.488
  pilF Neisseria gonorrhoeae MS11

49.12

98.611

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

47.509

97.569

0.464
  pilB Vibrio campbellii strain DS40M4

46.842

98.958

0.464

Multiple sequence alignment