Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NMV_RS00240 Genome accession   NC_017501
Coordinates   52402..53628 (-) Length   408 a.a.
NCBI ID   WP_002218483.1    Uniprot ID   Q9K1N4
Organism   Neisseria meningitidis 8013     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 47402..58628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMV_RS00235 (NMV_0046) yccS 49966..52116 (-) 2151 WP_014573685.1 YccS family putative transporter -
  NMV_RS00240 (NMV_0047) pilU 52402..53628 (-) 1227 WP_002218483.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMV_RS00245 (NMV_0048) pilT 53791..54834 (-) 1044 WP_002218487.1 type IV pilus twitching motility protein PilT Machinery gene
  NMV_RS00250 (NMV_0049) - 54954..55640 (+) 687 WP_002237503.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMV_RS00255 (NMV_0050) - 55640..56047 (+) 408 WP_002237504.1 hypothetical protein -
  NMV_RS00260 (NMV_0051) proC 56078..56887 (+) 810 WP_228372582.1 pyrroline-5-carboxylate reductase -
  NMV_RS00265 (NMV_0052) dksA 57035..57451 (+) 417 WP_002231430.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45674.28 Da        Isoelectric Point: 6.9640

>NTDB_id=38518 NMV_RS00240 WP_002218483.1 52402..53628(-) (pilU) [Neisseria meningitidis 8013]
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=38518 NMV_RS00240 WP_002218483.1 52402..53628(-) (pilU) [Neisseria meningitidis 8013]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAATGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCGCTGACAGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAGTCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTAGGCCTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCAGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCCGACGTGGTCTGCATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATCGACATCGCCC
TCAACCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAACAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(49-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9K1N4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

44.598

88.48

0.395
  pilU Pseudomonas stutzeri DSM 10701

44.663

87.255

0.39
  pilU Vibrio cholerae strain A1552

46.429

82.353

0.382

Multiple sequence alignment