Detailed information NTG0384823

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: MGAS2221_RS00675
• Genome accession: NZ_CP043530
• Coordinates: 104952..105386 (+)
• Length: 144 a.a.
• NCBI ID: WP_010921800.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain MGAS2221
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99952..110386

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MGAS2221_RS00645 (MGAS2221_0130)	-	101621..101986 (+)	366	WP_002986560.1	DUF1033 family protein	-
MGAS2221_RS00650 (MGAS2221_0131)	comYA	102079..103017 (+)	939	WP_010921798.1	competence type IV pilus ATPase ComGA	Machinery gene
MGAS2221_RS00655 (MGAS2221_0132)	comYB	102953..103987 (+)	1035	WP_011054115.1	competence type IV pilus assembly protein ComGB	Machinery gene
MGAS2221_RS00660 (MGAS2221_0133)	comYC	103989..104315 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
MGAS2221_RS00665 (MGAS2221_0134)	comGD	104290..104718 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
MGAS2221_RS00670 (MGAS2221_0135)	comGE	104675..104959 (+)	285	WP_002987779.1	competence type IV pilus minor pilin ComGE	-
MGAS2221_RS00675 (MGAS2221_0136)	comYF	104952..105386 (+)	435	WP_010921800.1	competence type IV pilus minor pilin ComGF	Machinery gene
MGAS2221_RS00680 (MGAS2221_0137)	comGG	105370..105696 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
MGAS2221_RS00685 (MGAS2221_0138)	comYH	105794..106747 (+)	954	WP_010921802.1	class I SAM-dependent methyltransferase	Machinery gene
MGAS2221_RS00690 (MGAS2221_0139)	-	106806..108002 (+)	1197	WP_010921803.1	acetate kinase	-
MGAS2221_RS00695	-	108189..108497 (+)	309	WP_010921804.1	hypothetical protein	-
MGAS2221_RS00700 (MGAS2221_0140)	proC	108580..109350 (-)	771	WP_010921805.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16654.28 Da
• Isoelectric Point: 10.2526

>NTDB_id=384823 MGAS2221_RS00675 WP_010921800.1 104952..105386(+) (comYF) [Streptococcus pyogenes strain MGAS2221]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTRTLLKHSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=384823 MGAS2221_RS00675 WP_010921800.1 104952..105386(+) (comYF) [Streptococcus pyogenes strain MGAS2221]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCGAACCCTCCTTAAACATAGCCATTATCTAGCCCGTCATGATCAAGATAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGGTTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	47.445	95.139	0.451
comGF/cglF	Streptococcus mitis NCTC 12261	44.03	93.056	0.41
comGF/cglF	Streptococcus pneumoniae Rx1	42.857	92.361	0.396
comGF/cglF	Streptococcus pneumoniae D39	42.857	92.361	0.396
comGF/cglF	Streptococcus pneumoniae R6	42.857	92.361	0.396
comGF/cglF	Streptococcus pneumoniae TIGR4	42.857	92.361	0.396
comGF/cglF	Streptococcus mitis SK321	42.105	92.361	0.389