Detailed information NTG0384169

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FZ919_RS14260
• Genome accession: NZ_CP043452
• Coordinates: 2955399..2956517 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain 18WIARLN0035
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2950399..2961517

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FZ919_RS14250 (FZ919_14365)	-	2953515..2954207 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
FZ919_RS14255 (FZ919_14370)	pilT	2954334..2955371 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
FZ919_RS14260 (FZ919_14375)	pilU	2955399..2956517 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FZ919_RS14265 (FZ919_14380)	fur	2956587..2957024 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
FZ919_RS14270 (FZ919_14385)	-	2957136..2957534 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
FZ919_RS14275 (FZ919_14390)	-	2957589..2957912 (-)	324	WP_000442587.1	RnfH family protein	-
FZ919_RS14280 (FZ919_14395)	-	2957912..2958979 (-)	1068	WP_002019572.1	hypothetical protein	-
FZ919_RS14285 (FZ919_14405)	-	2959132..2959587 (-)	456	WP_000782976.1	bacteriohemerythrin	-
FZ919_RS14290 (FZ919_14410)	-	2959840..2960688 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=384169 FZ919_RS14260 WP_000347039.1 2955399..2956517(+) (pilU) [Acinetobacter baumannii strain 18WIARLN0035]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=384169 FZ919_RS14260 WP_000347039.1 2955399..2956517(+) (pilU) [Acinetobacter baumannii strain 18WIARLN0035]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395