Detailed information NTG0380046

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FRD49_RS14155
• Genome accession: NZ_CP042994
• Coordinates: 2958384..2959502 (+)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0A1V0YUM2
• Organism: Acinetobacter nosocomialis strain J1A
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2953384..2964502

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FRD49_RS14140 (FRD49_14140)	-	2955550..2956392 (-)	843	WP_006581385.1	undecaprenyl-diphosphate phosphatase	-
FRD49_RS14145 (FRD49_14145)	-	2956500..2957192 (-)	693	WP_032010894.1	YggS family pyridoxal phosphate-dependent enzyme	-
FRD49_RS14150 (FRD49_14150)	pilT	2957319..2958356 (+)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
FRD49_RS14155 (FRD49_14155)	pilU	2958384..2959502 (+)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FRD49_RS14160 (FRD49_14160)	fur	2959572..2960009 (-)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
FRD49_RS14165 (FRD49_14165)	-	2960121..2960519 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
FRD49_RS14170 (FRD49_14170)	-	2960575..2960898 (-)	324	WP_032010899.1	RnfH family protein	-
FRD49_RS14175 (FRD49_14175)	-	2960898..2961965 (-)	1068	WP_004710227.1	hypothetical protein	-
FRD49_RS14185 (FRD49_14185)	-	2962118..2962573 (-)	456	WP_000782976.1	bacteriohemerythrin	-
FRD49_RS14190 (FRD49_14190)	-	2962825..2963673 (-)	849	WP_009505210.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=380046 FRD49_RS14155 WP_002052019.1 2958384..2959502(+) (pilU) [Acinetobacter nosocomialis strain J1A]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=380046 FRD49_RS14155 WP_002052019.1 2958384..2959502(+) (pilU) [Acinetobacter nosocomialis strain J1A]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCACCATC
AATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTAATTGGTCAACTCTTACATTCCA
TCATGAGTGAAAAACAACGTAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCCATCATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCGGGTATGGTACTACGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAACGTGAAGTCGGTATTGATACGGACTCTTTCGA
AATTGCTTTAAAAAATACATTACGACAAGCACCCGATGTCATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACAATGCACGCCAACAACGCCAACCAAGCACTCGATCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
GCAGCTTATTCCAACACCAGATGGCAATTCACGCCGTGCAGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTCGACTTATATAAAGCAGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAGCTTGCAAATGAAGGGCCTGATCAAATAACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A1V0YUM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403