Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   FRD49_RS01665 Genome accession   NZ_CP042994
Coordinates   350250..350990 (+) Length   246 a.a.
NCBI ID   WP_148356605.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain J1A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 345250..355990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FRD49_RS01650 (FRD49_01650) ponA 345830..348388 (-) 2559 WP_148356604.1 penicillin-binding protein PBP1a -
  FRD49_RS01655 (FRD49_01655) comM 348551..349609 (+) 1059 WP_004711654.1 pilus assembly protein PilM Machinery gene
  FRD49_RS01660 (FRD49_01660) pilN 349609..350253 (+) 645 WP_006581060.1 PilN domain-containing protein Machinery gene
  FRD49_RS01665 (FRD49_01665) pilO 350250..350990 (+) 741 WP_148356605.1 type 4a pilus biogenesis protein PilO Machinery gene
  FRD49_RS01670 (FRD49_01670) pilP 351001..351528 (+) 528 WP_006582542.1 pilus assembly protein PilP Machinery gene
  FRD49_RS01675 (FRD49_01675) pilQ 351591..353756 (+) 2166 WP_148356606.1 type IV pilus secretin PilQ Machinery gene
  FRD49_RS01680 (FRD49_01680) aroK 353768..354337 (+) 570 WP_002051689.1 shikimate kinase AroK -
  FRD49_RS01685 (FRD49_01685) aroB 354366..355448 (+) 1083 WP_002051763.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27635.44 Da        Isoelectric Point: 4.9673

>NTDB_id=380028 FRD49_RS01665 WP_148356605.1 350250..350990(+) (pilO) [Acinetobacter nosocomialis strain J1A]
MSQDELQELSLEQSASKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
GNLLNEFREKDSKLRNLQQYQVQLQEMQVNFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSISALPRIVTMHDFVVEAAQAKDSKSDIPVLNYSIKAKTYRYIGAPEASAQPAKAAAATAPTS
PNAQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=380028 FRD49_RS01665 WP_148356605.1 350250..350990(+) (pilO) [Acinetobacter nosocomialis strain J1A]
ATGAGTCAAGATGAACTGCAAGAATTATCATTAGAGCAATCAGCTTCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTTGATATGAACAACTATGGTAGTTGGCCAATATCTGTAAAAATTACTTGCTGGATTTTTATTT
TCTTTGCTGTATTGGCTTTGGGTTATTTTGTTGCTATTCAGCCAAAGCTACAAGCAATTGATAATGCCCAAGCTCAGGAA
GGTAATTTATTAAATGAATTTCGAGAAAAAGACTCCAAATTACGTAACCTTCAGCAGTATCAAGTACAGCTTCAAGAGAT
GCAGGTAAATTTCAACCAGCAATTAGAACAATTGCCGAAAGAAACAGAGATTCCAAGTTTAGTTGAGGATATTAACTTAA
CTGGTGTGAACTCTGGATTGAAATTTAAAAATATTCGTTTAGAGGATGAAGTAAAGCAGGAAATTTTTATTGAACAACCA
ATTGCCATCGAAGCAACAGGCGACTATCACGCTTTCGGTGCTTTCGTAAGTAGTATCTCTGCTTTGCCACGTATTGTGAC
GATGCATGACTTTGTTGTAGAGGCTGCTCAAGCTAAAGATAGTAAGTCAGATATTCCTGTGCTTAATTATTCTATTAAAG
CCAAAACCTATCGATATATAGGAGCACCAGAGGCATCGGCACAACCAGCTAAGGCTGCAGCGGCGACTGCACCAACTAGC
CCTAATGCTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

88.618

100

0.886
  comO Acinetobacter baylyi ADP1

70.539

97.967

0.691

Multiple sequence alignment