Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FWK48_RS08465 Genome accession   NZ_CP042987
Coordinates   1843217..1843675 (-) Length   152 a.a.
NCBI ID   WP_136121890.1    Uniprot ID   -
Organism   Histophilus somni strain UOC-KLM-ATR-08     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1838217..1848675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FWK48_RS08440 (FWK48_08440) - 1839864..1840115 (+) 252 WP_233120535.1 DUF5339 domain-containing protein -
  FWK48_RS08445 (FWK48_08445) artM 1840221..1840904 (-) 684 WP_011608408.1 arginine ABC transporter permease ArtM -
  FWK48_RS08450 (FWK48_08450) artQ 1840915..1841586 (-) 672 WP_136121889.1 arginine ABC transporter permease ArtQ -
  FWK48_RS08455 (FWK48_08455) - 1841591..1842316 (-) 726 WP_012340415.1 transporter substrate-binding domain-containing protein -
  FWK48_RS08460 (FWK48_08460) artP 1842334..1843071 (-) 738 WP_012340409.1 arginine ABC transporter ATP-binding protein ArtP -
  FWK48_RS08465 (FWK48_08465) pilA 1843217..1843675 (-) 459 WP_136121890.1 prepilin peptidase-dependent pilin Machinery gene
  FWK48_RS08470 (FWK48_08470) ampD 1843798..1844367 (+) 570 WP_075321738.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FWK48_RS08475 (FWK48_08475) cgtA 1844609..1845787 (-) 1179 WP_136121891.1 Obg family GTPase CgtA -
  FWK48_RS08480 (FWK48_08480) - 1845797..1846717 (-) 921 WP_075321736.1 DMT family transporter -
  FWK48_RS08485 (FWK48_08485) rpmA 1846853..1847110 (-) 258 WP_011608400.1 50S ribosomal protein L27 -
  FWK48_RS08490 (FWK48_08490) rplU 1847131..1847442 (-) 312 WP_011608399.1 50S ribosomal protein L21 -
  FWK48_RS08495 (FWK48_08495) ispB 1847683..1848654 (+) 972 WP_136121892.1 octaprenyl diphosphate synthase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15903.18 Da        Isoelectric Point: 8.4638

>NTDB_id=379871 FWK48_RS08465 WP_136121890.1 1843217..1843675(-) (pilA) [Histophilus somni strain UOC-KLM-ATR-08]
MKITYIFSSVKKGFTLIELMIVIAIVAILATIAIPSYQNYTKKAAITEILQASAPYRAEVELCVYNNDAPTNCNAGTNGI
QAAINTPKGKVKSVSVQGGAITVTGNGNLKDITYTLTPTGDASTGVTWSANCNNKADLFPAGFCSSTSTNSP

Nucleotide


Download         Length: 459 bp        

>NTDB_id=379871 FWK48_RS08465 WP_136121890.1 1843217..1843675(-) (pilA) [Histophilus somni strain UOC-KLM-ATR-08]
ATGAAAATAACCTATATTTTTTCATCAGTTAAAAAAGGATTTACTCTCATAGAACTGATGATTGTTATTGCTATTGTGGC
AATTCTTGCAACCATTGCAATTCCGTCATACCAAAATTATACAAAAAAAGCTGCAATTACAGAGATATTGCAAGCTTCAG
CTCCTTATCGTGCAGAAGTTGAGCTATGCGTGTATAACAATGATGCACCGACAAATTGTAATGCTGGAACAAATGGAATT
CAAGCGGCAATCAATACACCAAAAGGTAAAGTAAAATCAGTTAGCGTGCAAGGTGGAGCCATTACTGTTACAGGGAATGG
AAATCTTAAAGATATTACTTATACCTTAACACCAACAGGCGATGCTTCAACCGGCGTTACTTGGAGTGCAAATTGCAATA
ATAAGGCCGATCTATTTCCTGCCGGATTTTGCTCTTCTACGTCTACAAACTCTCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-33)

Pilin (bacterial filament) (Pilin)

(43-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

64.626

96.711

0.625
  pilA Haemophilus influenzae Rd KW20

62.585

96.711

0.605
  pilA Glaesserella parasuis strain SC1401

49.677

100

0.507
  pilA Ralstonia pseudosolanacearum GMI1000

35.714

100

0.395
  pilA Vibrio campbellii strain DS40M4

38.356

96.053

0.368

Multiple sequence alignment