Detailed information NTG0379040

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HB730_RS02345
• Genome accession: NZ_CP050332
• Coordinates: 505961..507109 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain DVT413
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 500961..512109

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB730_RS02320 (HB730_02330)	-	502112..502408 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HB730_RS02325 (HB730_02335)	-	502405..502998 (-)	594	WP_003084544.1	YggT family protein	-
HB730_RS02330 (HB730_02340)	proC	503009..503830 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HB730_RS02335 (HB730_02345)	-	503842..504534 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
HB730_RS02340 (HB730_02350)	pilT	504749..505783 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HB730_RS02345 (HB730_02355)	pilU	505961..507109 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
HB730_RS02350 (HB730_02360)	czcD	507117..508016 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HB730_RS02355 (HB730_02365)	-	508171..508575 (+)	405	WP_003084560.1	NINE protein	-
HB730_RS02360 (HB730_02370)	-	508818..510191 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HB730_RS02365 (HB730_02375)	-	510188..511372 (+)	1185	WP_058176557.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=379040 HB730_RS02345 WP_003110016.1 505961..507109(+) (pilU) [Pseudomonas aeruginosa strain DVT413]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=379040 HB730_RS02345 WP_003110016.1 505961..507109(+) (pilU) [Pseudomonas aeruginosa strain DVT413]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382