Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CS053_RS15115 Genome accession   NZ_CP042807
Coordinates   3404777..3405835 (-) Length   352 a.a.
NCBI ID   WP_008211562.1    Uniprot ID   I4W9K9
Organism   Rhodanobacter glycinis strain T01E-68     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3399777..3410835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS053_RS15095 (CS053_15095) pilQ 3400517..3402676 (-) 2160 WP_147628006.1 type IV pilus secretin PilQ -
  CS053_RS15100 (CS053_15100) - 3402732..3403271 (-) 540 WP_147628007.1 pilus assembly protein PilP -
  CS053_RS15105 (CS053_15105) pilO 3403268..3403939 (-) 672 WP_147628008.1 type 4a pilus biogenesis protein PilO -
  CS053_RS15110 (CS053_15110) - 3404007..3404777 (-) 771 WP_253260755.1 PilN domain-containing protein -
  CS053_RS15115 (CS053_15115) pilM 3404777..3405835 (-) 1059 WP_008211562.1 pilus assembly protein PilM Machinery gene
  CS053_RS15120 (CS053_15120) - 3406084..3408624 (+) 2541 WP_147628009.1 penicillin-binding protein 1A -
  CS053_RS15125 (CS053_15125) - 3408845..3409522 (+) 678 WP_147628010.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37952.39 Da        Isoelectric Point: 4.4882

>NTDB_id=378701 CS053_RS15115 WP_008211562.1 3404777..3405835(-) (pilM) [Rhodanobacter glycinis strain T01E-68]
MGLFTPKKPPLVGVDISSTAVKLLQLSQSGGRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRALARSGSKLKHAGAA
VAGSAVITRIVPMASELSEDDLEGQIQVEANQYIPYPIDEVSLDYEILGPVRDNPEMNNILLAASRTENVDMRVAALDLG
GLTAQVVDVEAFAMENAFALIADQLNVGRDALVAVIDIGATMTTLSVLRNQRTIYSREQVFGGKQLTDEIMRRYGLSYEE
AGRAKRKGGLPESYESEALEPFKESLVQQISRLLQFFYAGSEYSKVDQVVLAGGCASIEGIDGMLEEQLGVPCVVANPLA
RMSLSSRVQAQTLAQDAPALMIAVGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=378701 CS053_RS15115 WP_008211562.1 3404777..3405835(-) (pilM) [Rhodanobacter glycinis strain T01E-68]
GTGGGTCTCTTTACACCCAAGAAGCCGCCGCTGGTCGGCGTCGATATCAGTTCGACTGCCGTAAAGCTGCTGCAGCTCAG
TCAGTCTGGCGGCCGTTATCGCGTGGAACACTACGCGGTAGAGCCGCTGCCACCCAATGCCGTGGTCGAGAAGAACATCG
TCGAGGTCGAGGCGGTGGGCGAGGCGATCCGGCGCGCACTGGCGCGCTCGGGCTCCAAGCTCAAGCACGCGGGGGCGGCG
GTGGCCGGTTCGGCCGTCATCACGCGCATCGTGCCGATGGCCAGCGAGTTGTCGGAAGATGACCTGGAGGGTCAGATCCA
GGTCGAGGCCAACCAGTACATCCCGTATCCGATCGACGAGGTGAGTCTCGATTACGAGATCCTCGGCCCGGTACGCGACA
ACCCCGAGATGAACAACATCCTGCTTGCGGCCTCGCGTACCGAGAACGTCGACATGCGCGTCGCCGCGCTGGACCTGGGC
GGACTCACCGCGCAGGTGGTCGATGTCGAGGCCTTCGCGATGGAGAATGCCTTCGCCCTGATCGCCGACCAGCTCAACGT
GGGTCGCGACGCACTGGTGGCGGTGATCGACATCGGCGCCACCATGACCACGCTGTCGGTGCTGCGCAACCAGCGCACCA
TCTACTCGCGCGAGCAGGTGTTCGGCGGCAAGCAGCTTACCGACGAGATCATGCGTCGTTACGGACTCTCCTACGAGGAA
GCCGGCCGGGCCAAGCGCAAGGGCGGTCTGCCGGAATCCTACGAAAGCGAAGCGCTGGAGCCGTTCAAGGAATCGCTGGT
GCAGCAAATCAGTCGCCTGCTGCAGTTCTTCTACGCCGGTAGCGAATACAGCAAGGTCGACCAGGTGGTGCTGGCCGGCG
GTTGCGCCTCGATCGAAGGCATCGACGGCATGCTCGAAGAGCAGCTCGGCGTGCCGTGCGTGGTGGCCAATCCGCTGGCG
CGGATGTCGTTGTCCTCGCGCGTGCAGGCCCAGACGCTGGCCCAGGATGCGCCGGCGCTGATGATCGCGGTCGGACTCGC
GCTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4W9K9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.437

98.864

0.509
  comM Acinetobacter nosocomialis M2

51.149

98.864

0.506
  comM Acinetobacter baylyi ADP1

49.714

99.432

0.494
  pilM Legionella pneumophila strain ERS1305867

43.343

100

0.435

Multiple sequence alignment