Detailed information NTG0378475

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HB740_RS18110
• Genome accession: NZ_CP050325
• Coordinates: 3925573..3926721 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain DVT425
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3920573..3931721

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB740_RS18090 (HB740_18115)	-	3921311..3922495 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
HB740_RS18095 (HB740_18120)	-	3922492..3923865 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HB740_RS18100 (HB740_18125)	-	3924108..3924512 (-)	405	WP_003084560.1	NINE protein	-
HB740_RS18105 (HB740_18130)	czcD	3924666..3925565 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HB740_RS18110 (HB740_18135)	pilU	3925573..3926721 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HB740_RS18115 (HB740_18140)	pilT	3926899..3927933 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HB740_RS18120 (HB740_18145)	-	3928148..3928840 (+)	693	WP_031632112.1	YggS family pyridoxal phosphate-dependent enzyme	-
HB740_RS18125 (HB740_18150)	proC	3928852..3929673 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HB740_RS18130 (HB740_18155)	-	3929684..3930277 (+)	594	WP_003084544.1	YggT family protein	-
HB740_RS18135 (HB740_18160)	-	3930274..3930570 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=378475 HB740_RS18110 WP_003100959.1 3925573..3926721(-) (pilU) [Pseudomonas aeruginosa strain DVT425]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=378475 HB740_RS18110 WP_003100959.1 3925573..3926721(-) (pilU) [Pseudomonas aeruginosa strain DVT425]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382