Detailed information NTG0378414

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FR761_RS05635
• Genome accession: NZ_CP042556
• Coordinates: 1181640..1182758 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain E47
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1176640..1187758

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FR761_RS05600 (FR761_05600)	-	1177469..1178317 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
FR761_RS05605 (FR761_05605)	-	1178570..1179025 (+)	456	WP_000782976.1	bacteriohemerythrin	-
FR761_RS05615 (FR761_05615)	-	1179178..1180245 (+)	1068	WP_004835750.1	hypothetical protein	-
FR761_RS05620 (FR761_05620)	-	1180245..1180568 (+)	324	WP_000442589.1	RnfH family protein	-
FR761_RS05625 (FR761_05625)	-	1180623..1181021 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
FR761_RS05630 (FR761_05630)	fur	1181133..1181570 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
FR761_RS05635 (FR761_05635)	pilU	1181640..1182758 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FR761_RS05640 (FR761_05640)	pilT	1182786..1183823 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
FR761_RS05645 (FR761_05645)	-	1183950..1184642 (+)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=378414 FR761_RS05635 WP_000347036.1 1181640..1182758(-) (pilU) [Acinetobacter baumannii strain E47]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=378414 FR761_RS05635 WP_000347036.1 1181640..1182758(-) (pilU) [Acinetobacter baumannii strain E47]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392