Detailed information NTG0378197

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HB747_RS27340
• Genome accession: NZ_CP050322
• Coordinates: 5884196..5885344 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain DVT729
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5879196..5890344

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HB747_RS27320 (HB747_27365)	-	5879934..5881118 (-)	1185	WP_174707625.1	cystathionine gamma-synthase	-
HB747_RS27325 (HB747_27370)	-	5881115..5882488 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HB747_RS27330 (HB747_27375)	-	5882731..5883135 (-)	405	WP_003084560.1	NINE protein	-
HB747_RS27335 (HB747_27380)	czcD	5883289..5884188 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HB747_RS27340 (HB747_27385)	pilU	5884196..5885344 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HB747_RS27345 (HB747_27390)	pilT	5885522..5886556 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HB747_RS27350 (HB747_27395)	-	5886771..5887463 (+)	693	WP_174707626.1	YggS family pyridoxal phosphate-dependent enzyme	-
HB747_RS27355 (HB747_27400)	proC	5887475..5888296 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HB747_RS27360 (HB747_27405)	-	5888307..5888900 (+)	594	WP_003084544.1	YggT family protein	-
HB747_RS27365 (HB747_27410)	-	5888897..5889193 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=378197 HB747_RS27340 WP_003100959.1 5884196..5885344(-) (pilU) [Pseudomonas aeruginosa strain DVT729]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=378197 HB747_RS27340 WP_003100959.1 5884196..5885344(-) (pilU) [Pseudomonas aeruginosa strain DVT729]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTTGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382