Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   FGL73_RS02715 Genome accession   NZ_CP042408
Coordinates   567381..568703 (-) Length   440 a.a.
NCBI ID   WP_146977985.1    Uniprot ID   -
Organism   Lactococcus lactis strain CBA3619     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 562381..573703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL73_RS02685 (FGL73_02685) - 563523..564491 (+) 969 WP_146977982.1 PhoH family protein -
  FGL73_RS02690 (FGL73_02690) - 564549..565028 (+) 480 WP_146977983.1 NUDIX hydrolase -
  FGL73_RS02695 (FGL73_02695) ybeY 565180..565668 (+) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  FGL73_RS02700 (FGL73_02700) - 565652..566107 (+) 456 WP_003130653.1 diacylglycerol kinase family protein -
  FGL73_RS02705 (FGL73_02705) - 566121..566699 (+) 579 WP_146977984.1 nucleotidyltransferase family protein -
  FGL73_RS02710 (FGL73_02710) - 566734..567384 (-) 651 WP_058205632.1 ComF family protein -
  FGL73_RS02715 (FGL73_02715) comFA 567381..568703 (-) 1323 WP_146977985.1 DEAD/DEAH box helicase Machinery gene
  FGL73_RS02720 (FGL73_02720) - 568760..569389 (+) 630 WP_098393888.1 YigZ family protein -
  FGL73_RS02725 (FGL73_02725) - 569523..570020 (+) 498 WP_146977986.1 VanZ family protein -
  FGL73_RS02730 (FGL73_02730) - 570068..571087 (+) 1020 WP_038601219.1 DUF475 domain-containing protein -
  FGL73_RS02735 (FGL73_02735) - 571202..571948 (+) 747 WP_038601216.1 bifunctional metallophosphatase/5'-nucleotidase -
  FGL73_RS02740 (FGL73_02740) glyQ 572233..573186 (+) 954 WP_146977987.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50243.84 Da        Isoelectric Point: 10.1468

>NTDB_id=377694 FGL73_RS02715 WP_146977985.1 567381..568703(-) (comFA) [Lactococcus lactis strain CBA3619]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLIETTVKSILGMTSITSNKIKCNRCGTFHIKNAVKLPIGAFYC
PSCIQLGRVRSDEFLYFLPQKIFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILEK
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLVEQRNSGFPLLIFAAEIES
GQEFAKQLQLKFPKEKIAFVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=377694 FGL73_RS02715 WP_146977985.1 567381..568703(-) (comFA) [Lactococcus lactis strain CBA3619]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGATAGAAACAACGGTAAAATCAATTTTGGGGATGACTTCAATTACTT
CCAATAAAATAAAATGCAACCGCTGTGGGACTTTTCATATAAAAAATGCTGTCAAACTTCCAATTGGTGCATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTTCCACAAAAGATTTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGGGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAAA
GGTGGAGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GCTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAGGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGGAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTGCCGCGTCGTTTTCACAATTTTCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAGTTGAGCAAAGAAATAGTGGCTTTCCACTTCTAATTTTTGCTGCTGAAATTGAATCT
GGACAAGAATTTGCAAAACAACTACAACTAAAATTTCCTAAAGAAAAAATTGCTTTCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCTGCACTGATACAAATGGCTGGACGT
GTAGGTCGTAGTCCTGAGCGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(114-232)

Helicase conserved C-terminal domain (Helicase_C)

(301-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.5

90.909

0.677
  comFA/cflA Streptococcus pneumoniae Rx1

52.513

90.455

0.475
  comFA/cflA Streptococcus pneumoniae D39

52.513

90.455

0.475
  comFA/cflA Streptococcus pneumoniae R6

52.513

90.455

0.475
  comFA/cflA Streptococcus pneumoniae TIGR4

52.513

90.455

0.475
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

37.029

100

0.38

Multiple sequence alignment