Detailed information NTG0037732

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RPSI07_RS11050
• Genome accession: NC_014311
• Coordinates: 680290..681171 (-)
• Length: 293 a.a.
• NCBI ID: WP_048817321.1
• Uniprot ID: A0AAD0S7D3
• Organism: Ralstonia solanacearum PSI07
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 675290..686171

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RPSI07_RS11020 (RPSI07_0674)	argJ	675998..677227 (+)	1230	WP_013211398.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
RPSI07_RS11025 (RPSI07_0675)	-	677243..678127 (+)	885	WP_013211399.1	ATP-binding protein	-
RPSI07_RS11030 (RPSI07_0676)	-	678105..678563 (+)	459	WP_048817320.1	NUDIX domain-containing protein	-
RPSI07_RS11035 (RPSI07_0677)	-	678560..678775 (-)	216	WP_013211401.1	DNA gyrase inhibitor YacG	-
RPSI07_RS11040 (RPSI07_0678)	zapD	678787..679545 (-)	759	WP_013211402.1	cell division protein ZapD	-
RPSI07_RS11045 (RPSI07_0679)	coaE	679663..680277 (-)	615	WP_013211403.1	dephospho-CoA kinase	-
RPSI07_RS11050 (RPSI07_0680)	pilD	680290..681171 (-)	882	WP_048817321.1	prepilin peptidase	Machinery gene
RPSI07_RS11055 (RPSI07_0681)	pilC	681174..682439 (-)	1266	WP_013211405.1	type II secretion system F family protein	Machinery gene
RPSI07_RS11060 (RPSI07_0682)	pilF	682484..684211 (-)	1728	WP_013211406.1	type IV-A pilus assembly ATPase PilB	Machinery gene
RPSI07_RS11065 (RPSI07_0683)	-	684459..685736 (-)	1278	WP_013211407.1	HlyC/CorC family transporter	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31635.63 Da
• Isoelectric Point: 8.0336

>NTDB_id=37732 RPSI07_RS11050 WP_048817321.1 680290..681171(-) (pilD) [Ralstonia solanacearum PSI07]
MSGMFVIPTLAQLPAWFVIGSAALLGLLVGSFLNVVIHRLPRMIEREEANYIAELRDEPLPHPEPYNLVIPRSACPSCGH
QIKAVENIPVISWLALRGRCSACKAPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLVAGSMIDADTQLLPD
AITQPLLWLGLLVNLFDMFARLPDAVIGAMAGYLFLWTIYWAYKLLRGREGMGYGDFKLMAALGAWFGWQALPLLVLLSS
VVGLVFGAVRIARGAKSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Nucleotide

• Length: 882 bp

>NTDB_id=37732 RPSI07_RS11050 WP_048817321.1 680290..681171(-) (pilD) [Ralstonia solanacearum PSI07]
ATGTCCGGGATGTTTGTCATTCCCACGCTGGCGCAGTTGCCGGCGTGGTTCGTGATCGGCTCGGCGGCGCTGCTGGGCCT
GCTGGTCGGCAGCTTCCTCAACGTGGTGATCCACCGCCTGCCGCGCATGATCGAGCGCGAAGAGGCCAACTACATCGCCG
AGCTGCGCGACGAACCGCTGCCGCATCCCGAGCCGTACAACCTGGTCATACCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGGTCGAGAACATCCCGGTGATCAGCTGGCTGGCATTGCGCGGCCGCTGCAGCGCCTGCAAGGCGCCCAT
TTCCTGGCGCTACCCTGCCGTGGAGCTGGTGACCGGCGTGCTGACCGGTGCCTGCCTGTGGCATTTCGGGCCGACCTGGG
TAGCGGTGGCCTCGGCCGCGCTGCTGTGGTTCCTGGTCGCCGGCTCGATGATCGACGCCGACACGCAACTGCTGCCTGAT
GCCATCACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAATCTGTTCGACATGTTCGCGCGGCTGCCGGATGCGGTGAT
CGGCGCGATGGCCGGGTATCTGTTCCTGTGGACCATCTACTGGGCCTACAAGCTGCTGCGCGGCCGGGAAGGCATGGGCT
ACGGCGATTTCAAGCTGATGGCCGCGCTGGGTGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTGCTGCTGTCGTCG
GTGGTCGGTCTGGTGTTCGGGGCGGTGCGCATCGCGCGCGGCGCCAAGAGCGAATCGCCGTTTTCGTTCGGCCCGTTCAT
CGCGGGTGCCGGCGTCATCGCCCTGCTGGCCGGCCCGCAGCTGGTGGCATTGACGGGGCTCGCGCCGCTGCTGGCGCGGT
AA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

• Type IV leader peptidase family (Peptidase_A24) (140-247)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.208	90.444	0.481
pilD	Vibrio campbellii strain DS40M4	49.458	94.539	0.468
pilD	Neisseria gonorrhoeae MS11	48.881	91.468	0.447
pilD	Acinetobacter nosocomialis M2	45.865	90.785	0.416
pilD	Acinetobacter baumannii D1279779	45.865	90.785	0.416