Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AOLE_RS16950 Genome accession   NC_014259
Coordinates   3603846..3604646 (-) Length   266 a.a.
NCBI ID   WP_023274324.1    Uniprot ID   -
Organism   Acinetobacter oleivorans DR1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3598846..3609646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOLE_RS16930 (AOLE_16905) - 3599912..3600610 (-) 699 WP_004794712.1 tetratricopeptide repeat protein -
  AOLE_RS16935 (AOLE_16910) hisS 3600638..3601930 (-) 1293 WP_013198977.1 histidine--tRNA ligase -
  AOLE_RS16940 (AOLE_16915) ispG 3601927..3603042 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AOLE_RS16945 (AOLE_16920) - 3603064..3603855 (-) 792 WP_005302244.1 helix-turn-helix domain-containing protein -
  AOLE_RS16950 (AOLE_16925) pilF 3603846..3604646 (-) 801 WP_023274324.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AOLE_RS16955 (AOLE_16930) rlmN 3604662..3605897 (-) 1236 WP_005302240.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AOLE_RS16960 (AOLE_16935) ndk 3606021..3606452 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  AOLE_RS16965 (AOLE_16940) iscX 3606566..3606766 (-) 201 WP_003654869.1 Fe-S cluster assembly protein IscX -
  AOLE_RS16970 (AOLE_16945) - 3606866..3607417 (-) 552 WP_013198979.1 phosphatase PAP2 family protein -
  AOLE_RS16975 (AOLE_16950) - 3607442..3608731 (-) 1290 WP_081399209.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30176.45 Da        Isoelectric Point: 9.0417

>NTDB_id=37693 AOLE_RS16950 WP_023274324.1 3603846..3604646(-) (pilF) [Acinetobacter oleivorans DR1]
MSTPKLKTVLCMGVAVAFLVSGCQTTHVQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSELDNAQAHNNYGTYLYQMERYNDAIEQFRIAGTTLGYDQRYQALENLGRIYLKLGDV
TNAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=37693 AOLE_RS16950 WP_023274324.1 3603846..3604646(-) (pilF) [Acinetobacter oleivorans DR1]
TTGAGTACTCCAAAGTTAAAAACGGTATTATGTATGGGGGTTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TGTGCAAAAGAAAGACCCAGAAAAAGCGGTAAAAGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ATTCGGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCAACGGCAAATATGATGATGGGCATTTTA
CTACAGCAAGAAGGTAGTAAGCCGAACTTAGAAAAAGCAGAACATTACTTTAAACGAGCAATTTCATCTGAACTGGATAA
TGCTCAAGCACATAATAACTATGGTACGTACTTGTACCAAATGGAACGCTATAATGATGCGATCGAGCAATTTCGTATTG
CTGGTACAACATTAGGTTATGACCAACGCTATCAGGCGTTGGAAAATCTAGGGCGTATTTATTTAAAATTGGGTGATGTT
ACGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGATTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATACGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(89-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.609

100

0.936
  pilF Acinetobacter baylyi ADP1

75

97.744

0.733

Multiple sequence alignment