Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   FP481_RS12305 Genome accession   NZ_CP042157
Coordinates   2435303..2435800 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain B3-17D     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2430303..2440800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FP481_RS12280 (FP481_12280) gcvPB 2430465..2431937 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  FP481_RS12285 (FP481_12285) gcvPA 2431930..2433276 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  FP481_RS12290 (FP481_12290) gcvT 2433296..2434387 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  FP481_RS12295 (FP481_12295) - 2434546..2435070 (-) 525 WP_001015120.1 shikimate kinase -
  FP481_RS12300 (FP481_12300) - 2435060..2435206 (-) 147 WP_001792109.1 hypothetical protein -
  FP481_RS12305 (FP481_12305) comGF 2435303..2435800 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  FP481_RS12310 (FP481_12310) comGE 2435718..2436017 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  FP481_RS12315 (FP481_12315) comGD 2436004..2436450 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  FP481_RS12320 (FP481_12320) comGC 2436428..2436739 (-) 312 WP_000472253.1 competence type IV pilus major pilin ComGC Machinery gene
  FP481_RS12325 (FP481_12325) comGB 2436753..2437823 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  FP481_RS12330 (FP481_12330) comGA 2437795..2438769 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  FP481_RS12335 (FP481_12335) - 2438821..2439444 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  FP481_RS12340 (FP481_12340) - 2439441..2439770 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  FP481_RS12345 (FP481_12345) - 2439770..2440756 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=375784 FP481_RS12305 WP_001788897.1 2435303..2435800(-) (comGF) [Staphylococcus aureus strain B3-17D]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=375784 FP481_RS12305 WP_001788897.1 2435303..2435800(-) (comGF) [Staphylococcus aureus strain B3-17D]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment