Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G9V97_RS14870 Genome accession   NZ_CP049936
Coordinates   3176158..3177669 (+) Length   503 a.a.
NCBI ID   WP_106474751.1    Uniprot ID   -
Organism   Escherichia coli strain JL05     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3171158..3182669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G9V97_RS14840 (G9V97_14830) tusA 3171405..3171650 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  G9V97_RS14845 (G9V97_14835) zntA 3171752..3173950 (-) 2199 WP_000106510.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  G9V97_RS14850 (G9V97_14840) yhhN 3174024..3174650 (-) 627 WP_000964718.1 lysoplasmalogenase -
  G9V97_RS14855 (G9V97_14845) yhhM 3174791..3175150 (+) 360 WP_000042873.1 DUF2500 domain-containing protein -
  G9V97_RS14860 (G9V97_14850) yhhL 3175153..3175422 (-) 270 WP_001295207.1 DUF1145 family protein -
  G9V97_RS14865 (G9V97_14855) rsmD 3175412..3176008 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  G9V97_RS14870 (G9V97_14860) pilA 3176158..3177669 (+) 1512 WP_106474751.1 signal recognition particle-docking protein FtsY Machinery gene
  G9V97_RS14875 (G9V97_14865) ftsE 3177656..3178324 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  G9V97_RS14880 (G9V97_14870) ftsX 3178317..3179375 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  G9V97_RS14885 (G9V97_14875) rpoH 3179620..3180474 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  G9V97_RS14890 (G9V97_14880) livJ 3180745..3181848 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  G9V97_RS14895 (G9V97_14885) ataR 3181998..3182264 (+) 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55219.09 Da        Isoelectric Point: 4.1468

>NTDB_id=375768 G9V97_RS14870 WP_106474751.1 3176158..3177669(+) (pilA) [Escherichia coli strain JL05]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALLPERINNDSL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=375768 G9V97_RS14870 WP_106474751.1 3176158..3177669(+) (pilA) [Escherichia coli strain JL05]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAAACGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACAAGCCGTCGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAACCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAAGCGGTGATAGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTTGAAGAAATTGCCCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATCATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
CCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGGCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTGCCCGAGAGGATTAACAATGATTCGCTTTGA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.091

76.541

0.376

Multiple sequence alignment