Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FOY61_RS28945 Genome accession   NZ_CP041771
Coordinates   6023020..6024084 (-) Length   354 a.a.
NCBI ID   WP_023103573.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain A681     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6018020..6029084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOY61_RS28920 (FOY61_28920) aroK 6018567..6019085 (-) 519 WP_003095833.1 shikimate kinase AroK -
  FOY61_RS28925 (FOY61_28925) pilQ 6019097..6021229 (-) 2133 WP_003116137.1 type 4a pilus secretin PilQ Machinery gene
  FOY61_RS28930 (FOY61_28930) pilP 6021283..6021807 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP -
  FOY61_RS28935 (FOY61_28935) pilO 6021804..6022427 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO -
  FOY61_RS28940 (FOY61_28940) pilN 6022424..6023020 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  FOY61_RS28945 (FOY61_28945) pilM 6023020..6024084 (-) 1065 WP_023103573.1 type IV pilus assembly protein PilM Machinery gene
  FOY61_RS28950 (FOY61_28950) - 6024269..6026740 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -
  FOY61_RS28955 (FOY61_28955) - 6026845..6028113 (-) 1269 WP_003103278.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37964.43 Da        Isoelectric Point: 4.5399

>NTDB_id=374096 FOY61_RS28945 WP_023103573.1 6023020..6024084(-) (pilM) [Pseudomonas aeruginosa strain A681]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPEHVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=374096 FOY61_RS28945 WP_023103573.1 6023020..6024084(-) (pilM) [Pseudomonas aeruginosa strain A681]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCACGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACTGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAAGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.618

100

0.559
  comM Acinetobacter nosocomialis M2

55.618

100

0.559
  comM Acinetobacter baylyi ADP1

51.695

100

0.517
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment