Detailed information NTG0374016

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: FNS63_RS06665
• Genome accession: NZ_CP041770
• Coordinates: 1437506..1438120 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain DH8030
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1432506..1443120

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FNS63_RS06635 (FNS63_06630)	rpsT	1432806..1433072 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
FNS63_RS06640 (FNS63_06635)	holA	1433089..1434130 (-)	1042	Protein_1322	DNA polymerase III subunit delta	-
FNS63_RS06645 (FNS63_06640)	-	1434170..1434324 (-)	155	Protein_1323	hypothetical protein	-
FNS63_RS06650 (FNS63_06645)	-	1434365..1434499 (+)	135	WP_003152870.1	YqzM family protein	-
FNS63_RS06655 (FNS63_06650)	comEC	1434518..1436869 (-)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
FNS63_RS06660 (FNS63_06655)	-	1436870..1437439 (-)	570	WP_003152868.1	ComE operon protein 2	-
FNS63_RS06665 (FNS63_06660)	comEA	1437506..1438120 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
FNS63_RS06670 (FNS63_06665)	comER	1438179..1439000 (+)	822	WP_014305449.1	late competence protein ComER	-
FNS63_RS06675 (FNS63_06670)	-	1439069..1439806 (-)	738	WP_024085626.1	class I SAM-dependent methyltransferase	-
FNS63_RS06680 (FNS63_06675)	rsfS	1439803..1440159 (-)	357	WP_003152864.1	ribosome silencing factor	-
FNS63_RS06685 (FNS63_06680)	yqeK	1440177..1440737 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
FNS63_RS06690 (FNS63_06685)	-	1440727..1441296 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
FNS63_RS06695 (FNS63_06690)	yhbY	1441307..1441597 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
FNS63_RS06700 (FNS63_06695)	aroE	1441591..1442433 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=374016 FNS63_RS06665 WP_041482377.1 1437506..1438120(-) (comEA) [Bacillus amyloliquefaciens strain DH8030]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=374016 FNS63_RS06665 WP_041482377.1 1437506..1438120(-) (comEA) [Bacillus amyloliquefaciens strain DH8030]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363