Detailed information NTG0372385

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FK728_RS04595
• Genome accession: NZ_CP041587
• Coordinates: 966230..967348 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain J9
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 961230..972348

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FK728_RS04560 (FK728_00901)	-	962059..962907 (+)	849	WP_000336553.1	class II glutamine amidotransferase	-
FK728_RS04565 (FK728_00902)	-	963160..963615 (+)	456	WP_000782976.1	bacteriohemerythrin	-
FK728_RS04575 (FK728_00903)	-	963768..964835 (+)	1068	WP_001982581.1	membrane protein	-
FK728_RS04580 (FK728_00904)	-	964835..965158 (+)	324	WP_000442588.1	RnfH family protein	-
FK728_RS04585 (FK728_00905)	-	965213..965611 (-)	399	WP_001170993.1	outer membrane protein assembly factor BamE	-
FK728_RS04590 (FK728_00906)	fur	965723..966160 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
FK728_RS04595 (FK728_00907)	pilU	966230..967348 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FK728_RS04600 (FK728_00908)	pilT	967378..968415 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
FK728_RS04605 (FK728_00909)	-	968542..969234 (+)	693	WP_001108502.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=372385 FK728_RS04595 WP_000347036.1 966230..967348(-) (pilU) [Acinetobacter baumannii strain J9]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=372385 FK728_RS04595 WP_000347036.1 966230..967348(-) (pilU) [Acinetobacter baumannii strain J9]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAGGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACATTACGACAAGCGCCGGATGTCATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392