Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   IHMA87_RS25630 Genome accession   NZ_CP041354
Coordinates   5319189..5320781 (+) Length   530 a.a.
NCBI ID   WP_034081442.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AZPAE15042     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5314189..5325781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHMA87_RS25610 (IHMA87_05003) pgeF 5315942..5316670 (-) 729 WP_034081438.1 peptidoglycan editing factor PgeF -
  IHMA87_RS25615 (IHMA87_05004) rluD 5316667..5317629 (-) 963 WP_003150035.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  IHMA87_RS25620 (IHMA87_05005) - 5317775..5318800 (+) 1026 WP_012077305.1 outer membrane protein assembly factor BamD -
  IHMA87_RS25625 (IHMA87_05006) - 5318954..5319199 (+) 246 WP_003150030.1 PP0621 family protein -
  IHMA87_RS25630 (IHMA87_05007) pilS 5319189..5320781 (+) 1593 WP_034081442.1 two-component system sensor histidine kinase PilS Regulator
  IHMA87_RS25635 (IHMA87_05008) pilR 5320797..5322134 (+) 1338 WP_034081443.1 two-component system response regulator PilR Regulator
  IHMA87_RS25640 (IHMA87_05009) thiO 5322180..5323274 (-) 1095 WP_033999365.1 glycine oxidase ThiO -
  IHMA87_RS25645 (IHMA87_05010) - 5323409..5323918 (+) 510 WP_034081444.1 GspH/FimT family protein -
  IHMA87_RS25650 (IHMA87_05011) - 5324024..5324530 (+) 507 WP_012077308.1 Tfp pilus assembly protein FimT/FimU -
  IHMA87_RS25655 (IHMA87_05012) pilV 5324521..5325078 (+) 558 WP_034081446.1 type IV pilus modification protein PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59151.37 Da        Isoelectric Point: 7.0317

>NTDB_id=371462 IHMA87_RS25630 WP_034081442.1 5319189..5320781(+) (pilS) [Pseudomonas aeruginosa strain AZPAE15042]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEEQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLLLCGLFYAGGGMPSGIGSLLVVAVAIANILLRGRIGLVIAAVASLGLLYLTFFLSLSSPDAPNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSHLSILLANQAALGLLRQDQVRGASLD
RYSPMLVHCMKQWRLNPSRRPPTLKVIPEGPAVQPSFISLNREHDRHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDSADRRLTQIIQDQSRRMNLVIENVLQLSRRRQAEPQQLDLKDWLQRFVDEYPGKLRND
SELHLQLGAGDIHTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDADSDLPVLEIADDGPGVPTDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=371462 IHMA87_RS25630 WP_034081442.1 5319189..5320781(+) (pilS) [Pseudomonas aeruginosa strain AZPAE15042]
GTGCGCGCTGAGCGGCTGCGGCTGAGCGAGGAGCAAGGGCAGCGCATCCTCCGCCTGTATCACCTGTATCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAGCTGGAAGAACAGGTCCTGAAACTGGTCCATCCCGAACTGTTCCATG
TCGGCAGCTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCACGGCAATTGCTGCCGATCTTC
ATCCTCGCCCTCACCGACGTGCTGCTGCTCTGCGGCCTGTTCTACGCCGGCGGCGGCATGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCGATCGCCAACATCCTCCTGCGCGGGCGCATCGGCCTGGTGATCGCGGCGGTGGCCAGCCTGG
GCCTGCTGTACCTGACCTTCTTCCTCAGCCTGAGCAGCCCGGACGCCCCCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTGCGGCGCCAGGAACAGACGGAAACGCTGGCCGAGGAACG
CGCCGAGACGGTCGCCAACCTCGAAGAGCTCAACGCGCTGATCCTGCAGCGCATGCGCACCGGCATCCTGGTGGTCGACA
GCCACCTGAGCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACCAGGTGCGGGGCGCCAGCCTGGAC
CGCTACAGCCCGATGCTGGTGCACTGCATGAAGCAGTGGCGCCTCAACCCCAGTCGCCGCCCGCCGACCCTCAAGGTGAT
TCCGGAGGGCCCCGCCGTGCAGCCCAGCTTCATCAGCCTCAACCGTGAACACGACCGGCATGTGCTGATCTTCCTCGAAG
ACATCTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTCGCCGGCCTCGGCCGGCTGACCGCCGGCATCGCCCACGAG
ATCCGCAACCCGCTGGGCGCCATCAGTCACGCCGCGCAACTGCTGCAGGAGTCCGAGGAACTCGACAGCGCCGACCGCCG
CCTGACACAGATCATCCAGGACCAGTCGCGACGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGCCGCCGCC
AGGCCGAACCGCAGCAACTCGACCTGAAGGACTGGCTGCAACGCTTCGTCGACGAATATCCCGGCAAGCTGCGCAACGAC
AGCGAACTGCACCTGCAGCTCGGCGCCGGCGACATCCACACGCGCATGGACCCGCACCAGTTGAATCAGGTACTGAGCAA
CCTGGTGCAGAACGGCCTGCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGTCTCGCGCGCGATGCGG
ACAGCGACCTGCCGGTGCTGGAAATCGCCGACGACGGCCCCGGCGTGCCGACGGATAAGCTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAACTGTGCGAGAGCAACCAGGCGCGCAT
CGACTACCGCAATCGCGAGGAGGGTGGAGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

94.528

100

0.945

Multiple sequence alignment