Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FLM54_RS12930 Genome accession   NZ_CP041327
Coordinates   2785150..2786286 (-) Length   378 a.a.
NCBI ID   WP_138609270.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. Scap25     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2780150..2791286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLM54_RS12900 (FLM54_12955) - 2780268..2781161 (+) 894 WP_178956567.1 hypothetical protein -
  FLM54_RS12905 (FLM54_12960) - 2781239..2781712 (+) 474 WP_178956568.1 hypothetical protein -
  FLM54_RS12910 (FLM54_12965) rsmE 2781784..2782518 (+) 735 WP_178956569.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FLM54_RS12915 (FLM54_12970) gshB 2782585..2783532 (+) 948 WP_138609469.1 glutathione synthase -
  FLM54_RS12920 (FLM54_12975) - 2783655..2784212 (+) 558 WP_010391585.1 YqgE/AlgH family protein -
  FLM54_RS12925 (FLM54_12980) ruvX 2784496..2784945 (+) 450 WP_013465849.1 Holliday junction resolvase RuvX -
  FLM54_RS12930 (FLM54_12985) pilU 2785150..2786286 (-) 1137 WP_138609270.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FLM54_RS12935 (FLM54_12990) pilT 2786296..2787336 (-) 1041 WP_010391590.1 type IV pilus twitching motility protein PilT Machinery gene
  FLM54_RS12940 (FLM54_12995) - 2787339..2788052 (+) 714 WP_178956570.1 YggS family pyridoxal phosphate-dependent enzyme -
  FLM54_RS12945 (FLM54_13000) proC 2788070..2788891 (+) 822 WP_138609266.1 pyrroline-5-carboxylate reductase -
  FLM54_RS12950 (FLM54_13005) - 2788918..2789451 (+) 534 WP_178956571.1 YggT family protein -
  FLM54_RS12955 (FLM54_13010) - 2789510..2789953 (+) 444 WP_055012523.1 DUF4426 domain-containing protein -
  FLM54_RS12960 (FLM54_13015) - 2789956..2790555 (+) 600 WP_138569545.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41960.00 Da        Isoelectric Point: 6.2895

>NTDB_id=371203 FLM54_RS12930 WP_138609270.1 2785150..2786286(-) (pilU) [Pseudoalteromonas sp. Scap25]
MTLSLNHFLQIMIDKKGSDLFVSSQLPISAKINGELIPLNDDKLTDEQALELVESAMSEKQKAEFHNTKECNFAIATSEG
RFRISAFWQRDRAGMVIRRIVTQIPDVKELGLPSALTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHKKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTSDGEGRAAAIEVLLNTPMVSELIKNGNIGGIKETMAKSTEMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKGNIT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=371203 FLM54_RS12930 WP_138609270.1 2785150..2786286(-) (pilU) [Pseudoalteromonas sp. Scap25]
ATGACTTTATCTCTAAATCACTTTTTACAAATAATGATCGACAAAAAAGGCTCTGATCTATTTGTATCAAGCCAGCTGCC
TATTAGCGCTAAAATTAATGGCGAGCTGATACCTCTTAATGATGACAAGCTAACCGATGAGCAAGCGCTGGAATTAGTTG
AATCAGCGATGAGTGAAAAGCAAAAAGCAGAGTTTCATAACACCAAAGAGTGTAACTTTGCGATTGCAACCAGCGAAGGG
CGCTTTCGTATTTCGGCTTTTTGGCAACGCGATAGAGCGGGGATGGTTATTCGCCGGATTGTGACCCAAATACCTGATGT
AAAAGAACTTGGATTGCCCTCAGCGCTTACAGATGTGATTATGTCAAAGCGCGGACTGGTATTATTTGTCGGTGGTACGG
GAACCGGTAAATCAACGTCTTTGGCGGCGCTTATTGGTTATAGAAATCGCAATCAACGTGGGCATATTTTAACTATAGAA
GATCCAATAGAGTTTGTTCATGAACATAAAAAAAGCATTATTACTCAACGTGAAGTGGGCTTAGATACTGAAAGTTTTGA
GTCAGCACTAAAAAGCTCTTTACGCCAAGCGCCTGATGTTATTTTGATTGGTGAAATTCGATCGCAAGAAACTATGGAGT
ATGCGTTAAGCTTTGCAGAAACAGGTCATTTATGTGTTGCCACCTTACATGCTAACAATGCTAACCAAGCTATTGATCGT
ATTATGCACTTAGTTCCAAAAGAGAAGCACGATAAGCTTAAATATGATTTAGCATTAAACTTACGCGCTATTATTGCCCA
GCAGCTTATCCCTACCTCTGATGGTGAGGGACGTGCTGCTGCTATAGAGGTATTATTGAATACGCCAATGGTGAGTGAGC
TGATTAAAAACGGTAACATTGGTGGTATTAAAGAAACTATGGCCAAATCAACTGAAATGGGCATGCAGACCTTTGACCAA
GCGCTGTTTGAGCTTTACAGACAGCAACGGATTAACTATGCCGATGCACTTCATCATGCAGACTCGCCAAATGATTTACG
TTTAATGATAAAACTGCGTAATAACGAGCAGCAAGGGGCAGGCTTTTTGCAAGGTGTAACCATTGATGGCCTTGATGATA
AAGGTAATATTACCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

57.029

99.735

0.569
  pilU Pseudomonas stutzeri DSM 10701

57.333

99.206

0.569
  pilU Vibrio cholerae strain A1552

56.403

97.09

0.548
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.944

95.238

0.399

Multiple sequence alignment