Detailed information NTG0370764

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: FKV23_RS02965
• Genome accession: NZ_CP041242
• Coordinates: 692549..693607 (+)
• Length: 352 a.a.
• NCBI ID: WP_141622513.1
• Uniprot ID: A0A514BP58
• Organism: Marilutibacter alkalisoli strain SJ-36
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 687549..698607

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FKV23_RS17745 (FKV23_02945)	-	687633..687818 (-)	186	WP_141622509.1	hypothetical protein	-
FKV23_RS17750 (FKV23_02950)	-	688510..688887 (+)	378	WP_407067664.1	DUF4189 domain-containing protein	-
FKV23_RS02955 (FKV23_02955)	-	689028..689702 (-)	675	WP_141622511.1	hypothetical protein	-
FKV23_RS02960 (FKV23_02960)	-	689841..692321 (-)	2481	WP_141622512.1	penicillin-binding protein 1A	-
FKV23_RS02965 (FKV23_02965)	pilM	692549..693607 (+)	1059	WP_141622513.1	pilus assembly protein PilM	Machinery gene
FKV23_RS02970 (FKV23_02970)	-	693607..694380 (+)	774	WP_141622514.1	PilN domain-containing protein	-
FKV23_RS02975 (FKV23_02975)	-	694377..695033 (+)	657	WP_141622515.1	type 4a pilus biogenesis protein PilO	-
FKV23_RS02980 (FKV23_02980)	-	695092..695604 (+)	513	WP_141625010.1	pilus assembly protein PilP	-
FKV23_RS02985 (FKV23_02985)	-	695622..697556 (+)	1935	WP_141622516.1	type IV pilus secretin PilQ	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 37589.04 Da
• Isoelectric Point: 4.4714

>NTDB_id=370764 FKV23_RS02965 WP_141622513.1 692549..693607(+) (pilM) [Marilutibacter alkalisoli strain SJ-36]
MGVITKSQPALVGVDISSTAVKLLQLSRSGSRYRVEHYAVEPLPPNAVIEKNLVEVEAVGQAIKRAVSRSGTRARHAAAA
VAGSSVITKVIPMQADLDDDDMESAIELEAVNYVPYPIEEVNLDFEVLGPVPGSAETVQVLLAASRAENVEVRASALELG
GLIPKVMDVEAFAVENAFALIAEQLSGPRDGLVALIDAGATMTTLNVIRNGRSIYNREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYEVEVLEPFKEAMAQQVSRLLQFFYAGSEFNRVDQIVIAGGCASIPGIAQMVEEQLGVPTMVANPLA
NMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=370764 FKV23_RS02965 WP_141622513.1 692549..693607(+) (pilM) [Marilutibacter alkalisoli strain SJ-36]
GTGGGTGTCATCACAAAAAGCCAGCCGGCTCTCGTCGGCGTGGACATCAGTTCGACTGCAGTCAAGCTGCTGCAGCTGTC
GAGGTCCGGGAGTCGCTACCGCGTCGAGCATTACGCCGTGGAGCCACTGCCGCCGAACGCAGTGATCGAGAAGAACCTGG
TCGAGGTCGAGGCCGTCGGCCAGGCGATCAAGCGCGCCGTATCGCGGTCCGGGACCCGGGCCCGCCATGCCGCCGCCGCT
GTGGCGGGGTCGTCGGTGATCACCAAGGTGATCCCGATGCAGGCCGATCTGGACGACGACGACATGGAGTCGGCGATCGA
GCTGGAAGCCGTCAACTATGTGCCGTACCCGATCGAGGAAGTGAACCTCGACTTCGAGGTACTCGGCCCGGTGCCGGGCA
GCGCCGAGACCGTGCAGGTCCTGCTTGCCGCTTCGCGCGCCGAGAACGTCGAGGTCCGCGCCTCCGCGCTGGAACTGGGC
GGGCTGATCCCGAAGGTCATGGACGTGGAGGCGTTCGCGGTCGAGAACGCGTTCGCCCTGATTGCCGAGCAGCTGAGCGG
GCCGCGTGACGGCCTGGTCGCGCTGATCGATGCCGGCGCGACCATGACCACCCTCAACGTGATCCGCAACGGGCGCAGCA
TCTACAACCGCGAACAGGTGTTCGGCGGCAAGCAGCTGACTGACGAAGTCATGCGCCGCTACGGCCTGAGCTACGAGGAG
GCCGGCCTGGCCAAACGCCAGGGCGGATTGCCGGAAAGCTACGAGGTCGAGGTCCTGGAGCCGTTCAAGGAGGCGATGGC
CCAGCAGGTCAGCCGCCTGCTGCAGTTCTTCTACGCCGGCAGTGAGTTCAACCGTGTCGACCAGATCGTGATCGCCGGCG
GCTGCGCCTCGATCCCGGGCATCGCCCAGATGGTCGAGGAGCAACTGGGTGTGCCGACGATGGTCGCCAACCCGCTCGCG
AACATGACCCTCGGCCCACGTGTCCAGGCCCACGCCCTGGCGCAGGACGCGCCGGCGCTGATGATCGCCTGTGGCCTTGC
CTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-351)

3D structure

• AlphaFold DB: A0A514BP58

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	50.72	98.58	0.5
comM	Acinetobacter nosocomialis M2	50.432	98.58	0.497
comM	Acinetobacter baylyi ADP1	48.991	98.58	0.483
pilM	Legionella pneumophila strain ERS1305867	44.915	100	0.452