Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FY132_RS05465 Genome accession   NZ_CP048569
Coordinates   1089606..1091075 (+) Length   489 a.a.
NCBI ID   WP_262512290.1    Uniprot ID   -
Organism   Agrobacterium tumefaciens strain Gle001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1084606..1096075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FY132_RS05440 (FY132_05400) dinR/lexA 1084881..1085603 (-) 723 WP_099085450.1 transcriptional repressor LexA Regulator
  FY132_RS05445 (FY132_05405) - 1086401..1087417 (+) 1017 WP_144030928.1 PilN domain-containing protein -
  FY132_RS05450 (FY132_05410) gspM 1087414..1088010 (+) 597 WP_099085448.1 type II secretion system protein GspM -
  FY132_RS05455 (FY132_05415) - 1088007..1088576 (+) 570 WP_099085447.1 hypothetical protein -
  FY132_RS05460 (FY132_05420) - 1088566..1089462 (+) 897 WP_111817215.1 hypothetical protein -
  FY132_RS05465 (FY132_05425) pilB 1089606..1091075 (+) 1470 WP_262512290.1 GspE/PulE family protein Machinery gene
  FY132_RS05470 (FY132_05430) - 1091077..1092288 (+) 1212 WP_099085444.1 type II secretion system F family protein -
  FY132_RS05475 (FY132_05435) - 1092285..1092770 (+) 486 WP_099085443.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FY132_RS05480 (FY132_05440) - 1092802..1093131 (+) 330 WP_233283371.1 hypothetical protein -
  FY132_RS05485 (FY132_05445) - 1093128..1093772 (+) 645 WP_099085441.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FY132_RS05490 (FY132_05450) gspG 1093808..1094257 (-) 450 WP_099085440.1 type II secretion system major pseudopilin GspG -
  FY132_RS05495 (FY132_05455) - 1094265..1094717 (-) 453 WP_162686857.1 prepilin peptidase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 53289.00 Da        Isoelectric Point: 5.4053

>NTDB_id=369499 FY132_RS05465 WP_262512290.1 1089606..1091075(+) (pilB) [Agrobacterium tumefaciens strain Gle001]
MPEAELAQAIADFCMIGCAQSVFEADSEMLERMGTDFAARNALLPITGEKGALSLIVADPFSRPAIEAIEFLIGGRLPLI
VAPRSTIEEFFRVRKAVRDDGDFSGSHDVAGEEDVDRLLDIAKEAPVIRFVSRIIQRAVDGKATDIHIEPFEDIVRIRLR
TDGILQTLETVPRSMAAGITSRIKILSRLNIAERRLPQDGRLRASVRGQEIDFRVSVTPSVHGETVVMRILDRSNVRLEL
GALGFDEQATAAIAAITRKPNGIFLVTGPTGSGKTTTLYSILNDINDPRWKFFTVEDPVEYRMKGITQLQVDPGIGLTFA
SALRSVLRQDPDGILIGEIRDEETARIAVQAALTGHLVLSTLHTNSAAGAISRLRNMGIESYLLAATLRGIIGQRLVRRV
CEECRHHDSETMCAVCGGSGLAGRQAVYEIMEITDALRDSISRKDDENALEMAAQQVGMRSMREHAHGLVAAGVTTSGEV
SRVIDLQGA

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=369499 FY132_RS05465 WP_262512290.1 1089606..1091075(+) (pilB) [Agrobacterium tumefaciens strain Gle001]
ATGCCGGAAGCCGAACTGGCGCAGGCGATTGCAGATTTCTGCATGATCGGCTGCGCGCAATCGGTTTTCGAGGCAGACAG
TGAGATGCTGGAACGGATGGGTACGGATTTTGCAGCGAGGAATGCACTTCTGCCCATAACAGGAGAGAAAGGAGCGCTCT
CCCTGATCGTTGCGGACCCTTTTAGCCGCCCGGCCATAGAAGCGATCGAGTTTCTGATCGGGGGGCGTCTTCCGCTCATT
GTCGCGCCGAGATCGACGATTGAGGAGTTTTTTCGGGTTCGCAAGGCCGTGCGGGACGATGGTGATTTTTCCGGGTCCCA
TGACGTTGCAGGCGAGGAGGACGTTGATCGGTTGCTCGACATTGCGAAAGAGGCGCCGGTCATTCGCTTCGTATCCAGAA
TCATTCAGCGGGCCGTCGATGGCAAAGCGACGGATATTCATATCGAGCCTTTTGAGGATATTGTGAGAATTCGTTTGCGG
ACAGACGGCATTCTTCAAACCCTGGAGACCGTGCCGAGATCGATGGCGGCCGGTATAACGAGCCGGATCAAGATATTGTC
GCGGCTCAATATTGCGGAGCGACGCTTACCCCAGGACGGGCGGCTGCGGGCATCCGTTCGCGGGCAGGAGATCGATTTTC
GAGTCTCCGTGACGCCCAGCGTCCACGGCGAAACCGTTGTCATGCGAATTCTCGATCGCTCGAATGTGCGCCTCGAATTG
GGTGCCCTTGGATTTGATGAGCAGGCGACCGCGGCGATCGCGGCCATTACGCGCAAACCGAACGGGATATTTCTCGTCAC
CGGGCCAACGGGTTCCGGCAAGACGACGACACTCTACTCCATTCTAAACGACATCAACGATCCGAGGTGGAAGTTCTTCA
CGGTTGAAGATCCCGTCGAGTATCGGATGAAGGGAATTACCCAGTTGCAGGTCGATCCGGGGATTGGTCTGACCTTCGCC
TCAGCCCTGCGTTCAGTTCTCCGGCAGGACCCTGACGGCATCCTGATTGGGGAAATCCGAGACGAGGAGACGGCCAGAAT
CGCCGTGCAGGCGGCGCTGACCGGTCATCTTGTTCTATCGACGCTGCATACCAACAGCGCCGCCGGGGCTATCAGCCGGC
TTCGCAACATGGGGATCGAAAGCTACCTTCTTGCCGCAACTTTGCGGGGGATCATCGGCCAGAGGCTGGTTCGCAGGGTG
TGCGAGGAATGCCGGCATCACGACAGCGAGACGATGTGTGCCGTTTGTGGCGGCTCCGGTTTGGCCGGACGGCAAGCCGT
CTATGAGATCATGGAAATCACGGATGCGTTGCGAGATTCGATTTCCAGAAAAGACGACGAAAATGCCCTCGAAATGGCGG
CACAGCAGGTGGGTATGCGAAGCATGCGGGAGCATGCCCATGGGCTTGTTGCTGCCGGAGTGACGACCTCGGGCGAGGTT
TCGCGCGTCATCGACCTTCAGGGAGCCTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(3-92)

Type II/IV secretion system protein (T2SSE)

(134-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

38.246

100

0.419
  pilF Thermus thermophilus HB27

38.846

100

0.413
  pilB Legionella pneumophila strain ERS1305867

40.664

98.569

0.401
  pilB Vibrio campbellii strain DS40M4

36.704

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.915

96.319

0.384
  pilB Acinetobacter baumannii D1279779

38.351

99.182

0.38
  pilB Vibrio cholerae strain A1552

39.03

96.933

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

41.514

89.162

0.37