Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FY149_RS15625 Genome accession   NZ_CP048493
Coordinates   1077493..1078980 (+) Length   495 a.a.
NCBI ID   WP_262568944.1    Uniprot ID   -
Organism   Agrobacterium tumefaciens strain Tul001     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1072493..1083980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FY149_RS15600 (FY149_15510) dinR/lexA 1072786..1073508 (-) 723 WP_099085450.1 transcriptional repressor LexA Regulator
  FY149_RS15605 (FY149_15515) - 1074306..1075322 (+) 1017 WP_144030928.1 PilN domain-containing protein -
  FY149_RS15610 (FY149_15520) gspM 1075319..1075915 (+) 597 WP_099085448.1 type II secretion system protein GspM -
  FY149_RS15615 (FY149_15525) - 1075912..1076481 (+) 570 WP_099085447.1 hypothetical protein -
  FY149_RS15620 (FY149_15530) - 1076471..1077367 (+) 897 WP_111817215.1 hypothetical protein -
  FY149_RS15625 (FY149_15535) pilF 1077493..1078980 (+) 1488 WP_262568944.1 GspE/PulE family protein Machinery gene
  FY149_RS15630 (FY149_15540) - 1078982..1080193 (+) 1212 WP_099085444.1 type II secretion system F family protein -
  FY149_RS15635 (FY149_15545) - 1080190..1080675 (+) 486 WP_099085443.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FY149_RS15640 (FY149_15550) - 1080707..1081036 (+) 330 WP_233283371.1 hypothetical protein -
  FY149_RS15645 (FY149_15555) - 1081033..1081677 (+) 645 WP_099085441.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FY149_RS15650 (FY149_15560) gspG 1081713..1082162 (-) 450 WP_099085440.1 type II secretion system major pseudopilin GspG -
  FY149_RS15655 (FY149_15565) - 1082170..1082622 (-) 453 WP_162686857.1 prepilin peptidase -

Sequence

Protein


Download         Length: 495 a.a.        Molecular weight: 53988.86 Da        Isoelectric Point: 5.4116

>NTDB_id=368979 FY149_RS15625 WP_262568944.1 1077493..1078980(+) (pilF) [Agrobacterium tumefaciens strain Tul001]
MRELGLMPEAELAQAIADFCMIGCAQSVFEADSEMLERMGTDFAARNALLPITGEKGALSLIVADPFSRPAIEAIEFLIG
GRLPLIVAPRSTIEEFFRVRKAVRDDGDFSGSHDVAGEEDVDRLLDIAKEAPVIRFVSRIIQRAVDGKATDIHIEPFEDI
VRIRLRTDGILQTLETVPRSMAAGITSRIKILSRLNIAERRLPQDGRLRASVRGQEIDFRVSVTPSVHGETVVMRILDRS
NVRLELGALGFDEQATAAIAAITRKPNGIFLVTGPTGSGKTTTLYSILNDINDPRWKFFTVEDPVEYRMKGITQLQVDPG
IGLTFASALRSVLRQDPDGILIGEIRDEETARIAVQAALTGHLVLSTLHTNSAAGAISRLRNMGIESYLLAATLRGIIGQ
RLVRRVCEECRHHDSETMCAVCGGSGLAGRQAVYEIMEITDALRDSISRKDDENALEMAAQQVGMRSMREHAHGLVAAGV
TTSGEVSRVIDLQGA

Nucleotide


Download         Length: 1488 bp        

>NTDB_id=368979 FY149_RS15625 WP_262568944.1 1077493..1078980(+) (pilF) [Agrobacterium tumefaciens strain Tul001]
ATGCGTGAGCTCGGGCTGATGCCGGAAGCCGAACTGGCGCAGGCGATTGCAGATTTCTGCATGATCGGCTGCGCGCAATC
GGTTTTCGAGGCAGACAGTGAGATGCTGGAACGGATGGGTACGGATTTTGCAGCGAGGAATGCACTTCTGCCCATAACAG
GAGAGAAAGGAGCGCTCTCCCTGATCGTTGCGGACCCTTTTAGCCGCCCGGCCATAGAAGCGATCGAGTTTCTGATCGGG
GGGCGTCTTCCGCTCATTGTCGCGCCGAGATCGACGATTGAGGAGTTTTTTCGGGTTCGCAAGGCCGTGCGGGACGATGG
TGATTTTTCCGGGTCCCATGACGTTGCAGGCGAGGAGGACGTTGATCGGTTGCTCGACATTGCGAAAGAGGCGCCGGTCA
TTCGCTTCGTATCCAGAATCATTCAGCGGGCCGTCGATGGCAAAGCGACGGATATTCATATCGAGCCTTTTGAGGATATT
GTGAGAATTCGTTTGCGGACAGACGGCATTCTTCAAACCCTGGAGACCGTGCCGAGATCGATGGCGGCCGGTATAACGAG
CCGGATCAAGATATTGTCGCGGCTCAATATTGCGGAGCGACGCTTACCCCAGGACGGGCGGCTGCGGGCATCCGTTCGCG
GGCAGGAGATCGATTTTCGAGTCTCCGTGACGCCCAGCGTCCACGGCGAAACCGTTGTCATGCGAATTCTCGATCGCTCG
AATGTGCGCCTCGAATTGGGTGCCCTTGGATTTGATGAGCAGGCGACCGCGGCGATCGCGGCCATTACGCGCAAACCGAA
CGGGATATTTCTCGTCACCGGGCCAACGGGTTCCGGCAAGACGACGACACTCTACTCCATTCTAAACGACATCAACGATC
CGAGGTGGAAGTTCTTCACGGTTGAAGATCCCGTCGAGTATCGGATGAAGGGAATTACCCAGTTGCAGGTCGATCCGGGG
ATTGGTCTGACCTTCGCCTCAGCCCTGCGTTCAGTTCTCCGGCAGGACCCTGACGGCATCCTGATTGGGGAAATCCGAGA
CGAGGAGACGGCCAGAATCGCCGTGCAGGCGGCGCTGACCGGTCATCTTGTTCTATCGACGCTGCATACCAACAGCGCCG
CCGGGGCTATCAGCCGGCTTCGCAACATGGGGATCGAAAGCTACCTTCTTGCCGCAACTTTGCGGGGGATCATCGGCCAG
AGGCTGGTTCGCAGGGTGTGCGAGGAATGCCGGCATCACGACAGCGAGACGATGTGTGCCGTTTGTGGCGGCTCCGGTTT
GGCCGGACGGCAAGCCGTCTATGAGATCATGGAAATCACGGATGCGTTGCGAGATTCGATTTCCAGAAAAGACGACGAAA
ATGCCCTCGAAATGGCGGCACAGCAGGTGGGTATGCGAAGCATGCGGGAGCATGCCCATGGGCTTGTTGCTGCCGGAGTG
ACGACCTCGGGCGAGGTTTCGCGCGTCATCGACCTTCAGGGAGCCTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(140-405)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(2-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.889

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

38.258

100

0.408
  pilB Legionella pneumophila strain ERS1305867

40.664

97.374

0.396
  pilB Vibrio campbellii strain DS40M4

36.692

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.915

95.152

0.38
  pilB Acinetobacter baumannii D1279779

38.351

97.98

0.376
  pilB Vibrio cholerae strain A1552

39.03

95.758

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

41.514

88.081

0.366

Multiple sequence alignment