Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   GZH78_RS03115 Genome accession   NZ_CP048408
Coordinates   688673..689296 (+) Length   207 a.a.
NCBI ID   WP_074689561.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain DR397     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 683673..694296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH78_RS03100 (GZH78_03100) - 684381..686828 (-) 2448 WP_039766412.1 penicillin-binding protein 1A -
  GZH78_RS03105 (GZH78_03105) pilM 687046..688110 (+) 1065 WP_039766420.1 pilus assembly protein PilM Machinery gene
  GZH78_RS03110 (GZH78_03110) - 688110..688676 (+) 567 WP_074689559.1 PilN domain-containing protein -
  GZH78_RS03115 (GZH78_03115) pilO 688673..689296 (+) 624 WP_074689561.1 type 4a pilus biogenesis protein PilO Machinery gene
  GZH78_RS03120 (GZH78_03120) pilP 689293..689817 (+) 525 WP_074689563.1 pilus assembly protein PilP Machinery gene
  GZH78_RS03125 (GZH78_03125) pilQ 689832..691910 (+) 2079 WP_039766429.1 type IV pilus secretin PilQ Machinery gene
  GZH78_RS03130 (GZH78_03130) aroK 691915..692433 (+) 519 WP_011332081.1 shikimate kinase AroK -
  GZH78_RS03135 (GZH78_03135) aroB 692586..693686 (+) 1101 WP_074689567.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 23149.60 Da        Isoelectric Point: 4.4691

>NTDB_id=368570 GZH78_RS03115 WP_074689561.1 688673..689296(+) (pilO) [Pseudomonas fluorescens strain DR397]
MKPSEWLESLRSVDFNDLDTSNIGSWPPVVKSLGGGLLMVLVLALGYNFFISDLENQLELKREEEITLKEQFASKARMAA
NLELYTQQMKEMENSFGVLLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQITVTGAYHDLATF
VSGVAGLPRIVTLHDFELAPANPEGGPKLRMSILAKTYRYNDKGLQK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=368570 GZH78_RS03115 WP_074689561.1 688673..689296(+) (pilO) [Pseudomonas fluorescens strain DR397]
ATGAAGCCGTCCGAGTGGCTGGAAAGCCTGCGCAGTGTCGATTTCAACGACCTCGACACCAGCAACATCGGCTCCTGGCC
GCCAGTGGTCAAAAGCCTGGGCGGCGGGCTGTTGATGGTGCTGGTCCTGGCGCTTGGCTATAACTTTTTCATCAGTGACC
TGGAGAACCAGCTCGAACTCAAACGTGAGGAGGAAATCACCCTCAAGGAGCAGTTCGCGAGCAAGGCGCGCATGGCGGCC
AATCTGGAGCTGTACACCCAGCAGATGAAAGAGATGGAAAACTCGTTCGGCGTGCTGTTGCGACAACTGCCCAGTGACAC
CGAAGTGCCGGGGTTGCTGGAAGACATCACCCGCACCGGACTGGGCAGTGGCCTTGAGTTCGAAGAGATCAAACTGCTGC
CTGAGGTTACCCAGCAGTTCTACATCGAGCTGCCCATCCAGATCACCGTGACCGGGGCCTATCACGACCTCGCGACCTTC
GTCAGTGGCGTGGCCGGGCTGCCACGGATCGTCACCCTGCACGATTTCGAGCTGGCGCCAGCCAATCCTGAAGGCGGGCC
GAAGCTGCGCATGAGCATCCTCGCCAAGACCTACCGCTATAACGACAAGGGGCTGCAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(59-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

45.263

91.787

0.415
  comO/pilO Acinetobacter baylyi ADP1

44.974

91.304

0.411
  pilO Legionella pneumophila strain ERS1305867

42.857

94.686

0.406