Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GZS15_RS03830 Genome accession   NZ_CP048322
Coordinates   801245..802738 (+) Length   497 a.a.
NCBI ID   WP_000249360.1    Uniprot ID   -
Organism   Escherichia coli strain 64     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 796245..807738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZS15_RS03810 (GZS15_03815) pilD 796893..797702 (+) 810 WP_074684053.1 prepilin peptidase PppA Machinery gene
  GZS15_RS03815 (GZS15_03820) gspS2 797768..798178 (+) 411 WP_001349452.1 type II secretion system pilot lipoprotein GspS-beta -
  GZS15_RS03820 (GZS15_03825) gspC 798196..799155 (+) 960 WP_074684050.1 type II secretion system protein GspC -
  GZS15_RS03825 (GZS15_03830) gspD 799185..801245 (+) 2061 WP_000498824.1 type II secretion system secretin GspD -
  GZS15_RS03830 (GZS15_03835) pilB/pilB1 801245..802738 (+) 1494 WP_000249360.1 type II secretion system ATPase GspE Machinery gene
  GZS15_RS03835 (GZS15_03840) gspF 802738..803961 (+) 1224 WP_001173453.1 type II secretion system inner membrane protein GspF -
  GZS15_RS03840 (GZS15_03845) gspG 803978..804433 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  GZS15_RS03845 (GZS15_03850) gspH 804437..805000 (+) 564 WP_001115140.1 type II secretion system minor pseudopilin GspH -
  GZS15_RS03850 (GZS15_03855) gspI 804997..805368 (+) 372 WP_000820125.1 type II secretion system minor pseudopilin GspI -
  GZS15_RS03855 (GZS15_03860) gspJ 805365..805964 (+) 600 WP_086732157.1 type II secretion system minor pseudopilin GspJ -
  GZS15_RS03860 (GZS15_03865) gspK 805967..806944 (+) 978 WP_000633238.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55203.37 Da        Isoelectric Point: 6.1634

>NTDB_id=367766 GZS15_RS03830 WP_000249360.1 801245..802738(+) (pilB/pilB1) [Escherichia coli strain 64]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDNAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=367766 GZS15_RS03830 WP_000249360.1 801245..802738(+) (pilB/pilB1) [Escherichia coli strain 64]
ATGGTGCCTGTAGCACAGGAAACCACCGCCAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCGGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACAATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGTTGCTGGGCGGCCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGGCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGCCCGCACCAGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.615

79.879

0.388
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.387

86.72

0.376
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

45.5

80.483

0.366