Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GWK05_RS04415 Genome accession   NZ_CP048107
Coordinates   933181..934674 (+) Length   497 a.a.
NCBI ID   WP_023141654.1    Uniprot ID   -
Organism   Escherichia coli strain 201609     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 928181..939674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK05_RS04395 (GWK05_04390) pilD 928829..929638 (+) 810 WP_001296399.1 prepilin peptidase PppA Machinery gene
  GWK05_RS04400 (GWK05_04395) gspS2 929704..930114 (+) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  GWK05_RS04405 (GWK05_04400) gspC 930132..931091 (+) 960 WP_023141653.1 type II secretion system protein GspC -
  GWK05_RS04410 (GWK05_04405) gspD 931121..933181 (+) 2061 WP_000498848.1 type II secretion system secretin GspD -
  GWK05_RS04415 (GWK05_04410) pilB/pilB1 933181..934674 (+) 1494 WP_023141654.1 type II secretion system ATPase GspE Machinery gene
  GWK05_RS04420 (GWK05_04415) gspF 934674..935897 (+) 1224 WP_061092721.1 type II secretion system inner membrane protein GspF -
  GWK05_RS04425 (GWK05_04420) gspG 935914..936369 (+) 456 WP_001087298.1 type II secretion system major pseudopilin GspG -
  GWK05_RS04430 (GWK05_04425) gspH 936373..936936 (+) 564 WP_024175821.1 type II secretion system minor pseudopilin GspH -
  GWK05_RS04435 (GWK05_04430) gspI 936933..937304 (+) 372 WP_000820092.1 type II secretion system minor pseudopilin GspI -
  GWK05_RS04440 (GWK05_04435) gspJ 937301..937906 (+) 606 WP_001254778.1 type II secretion system minor pseudopilin GspJ -
  GWK05_RS04445 (GWK05_04440) gspK 937903..938880 (+) 978 WP_001598627.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55163.23 Da        Isoelectric Point: 5.9800

>NTDB_id=367151 GWK05_RS04415 WP_023141654.1 933181..934674(+) (pilB/pilB1) [Escherichia coli strain 201609]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLMGISDTHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=367151 GWK05_RS04415 WP_023141654.1 933181..934674(+) (pilB/pilB1) [Escherichia coli strain 201609]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAACTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGCAAGCTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
ACAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATCGACGTGCGCGTCTCCACCATGCCTTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACACTGGAGCGTCTGGGTTTAAGTCACGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACAGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCGTCGCTCACGGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACACGTCTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGGCTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGGCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTTATGGGGATCTCCGATACGCATGCCGTCACGCTGTACCATCCGCAGGGTTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTACGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAGCGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.926

100

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Legionella pneumophila strain ERS1305867

41.97

93.964

0.394
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

44.75

80.483

0.36