Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   GV831_RS10540 Genome accession   NZ_CP047891
Coordinates   2138739..2139812 (+) Length   357 a.a.
NCBI ID   WP_014570824.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain WHH2311     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2133739..2144812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GV831_RS10535 (GV831_10495) comGA 2137906..2138845 (+) 940 Protein_2034 competence type IV pilus ATPase ComGA -
  GV831_RS10540 (GV831_10500) comGB 2138739..2139812 (+) 1074 WP_014570824.1 competence type IV pilus assembly protein ComGB Machinery gene
  GV831_RS10545 (GV831_10505) - 2139826..2139966 (+) 141 WP_228777719.1 hypothetical protein -
  GV831_RS10550 (GV831_10510) - 2139963..2141420 (-) 1458 WP_014570823.1 recombinase family protein -
  GV831_RS10555 (GV831_10515) - 2141546..2142085 (-) 540 WP_014570822.1 PH domain-containing protein -
  GV831_RS10560 (GV831_10520) - 2142141..2142725 (-) 585 WP_014570821.1 hypothetical protein -
  GV831_RS10565 (GV831_10525) - 2142736..2143146 (-) 411 WP_014570820.1 helix-turn-helix domain-containing protein -
  GV831_RS10570 (GV831_10530) - 2143323..2143556 (+) 234 WP_014570819.1 helix-turn-helix transcriptional regulator -
  GV831_RS10575 (GV831_10535) - 2143615..2144304 (+) 690 WP_014570818.1 phage regulatory protein -
  GV831_RS10580 (GV831_10540) - 2144320..2144502 (+) 183 WP_043991177.1 hypothetical protein -
  GV831_RS10585 - 2144499..2144621 (+) 123 WP_014570816.1 hypothetical protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40366.59 Da        Isoelectric Point: 10.4062

>NTDB_id=366436 GV831_RS10540 WP_014570824.1 2138739..2139812(+) (comGB) [Lactococcus lactis subsp. lactis strain WHH2311]
MSLGHQKIGIKKLISYLQMDISHLLKPRGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKKFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=366436 GV831_RS10540 WP_014570824.1 2138739..2139812(+) (comGB) [Lactococcus lactis subsp. lactis strain WHH2311]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAGAGGGAAAAAATTAAGGCTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAAAATTTACATTGAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTGGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACCTTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

82.698

95.518

0.79
  comGB Streptococcus gordonii str. Challis substr. CH1

53.666

95.518

0.513
  comGB Streptococcus mutans UA140

53.372

95.518

0.51
  comGB Streptococcus mutans UA159

53.079

95.518

0.507
  comGB/cglB Streptococcus pneumoniae TIGR4

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae D39

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae R6

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae Rx1

51.343

93.838

0.482
  comGB/cglB Streptococcus mitis SK321

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.149

93.838

0.471

Multiple sequence alignment