Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FEO84_RS18325 Genome accession   NZ_CP040440
Coordinates   3894795..3896528 (-) Length   577 a.a.
NCBI ID   WP_012481096.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain ICU331     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3889795..3901528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEO84_RS18310 (FEO84_18465) sucC 3890095..3891264 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  FEO84_RS18315 (FEO84_18470) - 3891622..3893235 (+) 1614 WP_049396998.1 ATP-binding protein -
  FEO84_RS18320 (FEO84_18475) pilR 3893296..3894669 (+) 1374 WP_005410795.1 sigma-54 dependent transcriptional regulator Regulator
  FEO84_RS18325 (FEO84_18480) pilB 3894795..3896528 (-) 1734 WP_012481096.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FEO84_RS18330 (FEO84_18485) - 3896751..3897158 (-) 408 WP_012481097.1 pilin -
  FEO84_RS18335 (FEO84_18490) - 3897311..3897733 (-) 423 WP_012481098.1 pilin -
  FEO84_RS18340 (FEO84_18495) pilC 3898087..3899346 (+) 1260 WP_012481099.1 type II secretion system F family protein Machinery gene
  FEO84_RS18345 (FEO84_18500) - 3899354..3900217 (+) 864 WP_012481100.1 A24 family peptidase -
  FEO84_RS18350 (FEO84_18505) coaE 3900229..3900840 (+) 612 WP_012481101.1 dephospho-CoA kinase -
  FEO84_RS18355 (FEO84_18510) - 3901033..3901350 (-) 318 WP_229302602.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62405.32 Da        Isoelectric Point: 5.8722

>NTDB_id=364458 FEO84_RS18325 WP_012481096.1 3894795..3896528(-) (pilB) [Stenotrophomonas maltophilia strain ICU331]
MNAVTTANLVGITGLARRLVQDGALDETTARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGRLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGATGSDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPSSLPE
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=364458 FEO84_RS18325 WP_012481096.1 3894795..3896528(-) (pilB) [Stenotrophomonas maltophilia strain ICU331]
ATGAACGCTGTCACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGACGCCTGGTACAGGACGGCGCCCTGGACGA
GACCACGGCCCGCGACGCCATGGCCAAGGCCGCCGCCGCGCGCCAGCCACTGCCGACCTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACCTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGTGGCAGGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCATACCAACCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGGCGCACGCTGGAACAGTGGCACGCCAGCCATGACACCCTCGGCGACGCCCTGGGTGGCGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTCGGCGCCACCGGCAGCGATTCCGGCATCGACGC
CAAGGGTGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCCTCGGACATCC
ACTTCGAGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGCGCGCCGGTG
AAGCTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTCCCGCAGGACGG
CCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTG
TTCCTGGATGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACTGGCCCGACCGGATCGGGCAAGACCGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCG
TCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCCTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGCCTGTGCGGCAACTGCAAGCGTCCGTCCAGCCTGCCCGAA
CATGCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCGAGCTGTACGAGGCCGTGGGCTGCGACGA
ATGCACCGAGGGCTACAAGGGCCGGACCGGCGTCTACCAGGTCATGCCGATGACCGATGAGATCGCTACCATCGTGCTGG
CCGGTGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGCCAGTCGGCATTGAAGAAG
GCGGCGGCGGGCATCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.872

100

0.556
  pilB Acinetobacter baumannii D1279779

55.36

98.614

0.546
  pilB Legionella pneumophila strain ERS1305867

52.105

98.787

0.515
  pilB Vibrio cholerae strain A1552

48.342

99.307

0.48
  pilF Neisseria gonorrhoeae MS11

48.596

98.787

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

45.629

99.133

0.452
  pilB Vibrio campbellii strain DS40M4

45.343

98.614

0.447

Multiple sequence alignment