Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FEO95_RS18525 Genome accession   NZ_CP040432
Coordinates   4028758..4029816 (-) Length   352 a.a.
NCBI ID   WP_006388916.1    Uniprot ID   B4STX6
Organism   Stenotrophomonas maltophilia strain sm-RA9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4023758..4034816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEO95_RS18505 (FEO95_18600) - 4024699..4026672 (-) 1974 WP_006388908.1 type IV pilus secretin PilQ family protein -
  FEO95_RS18510 (FEO95_18605) - 4026694..4027197 (-) 504 WP_050559910.1 pilus assembly protein PilP -
  FEO95_RS18515 (FEO95_18610) pilO 4027224..4027880 (-) 657 WP_006388913.1 type 4a pilus biogenesis protein PilO -
  FEO95_RS18520 (FEO95_18615) - 4027877..4028758 (-) 882 WP_006388915.1 PilN domain-containing protein -
  FEO95_RS18525 (FEO95_18620) pilM 4028758..4029816 (-) 1059 WP_006388916.1 pilus assembly protein PilM Machinery gene
  FEO95_RS18530 (FEO95_18625) - 4030036..4032459 (+) 2424 WP_154354815.1 penicillin-binding protein 1A -
  FEO95_RS18535 (FEO95_18630) - 4032799..4033443 (+) 645 WP_012511978.1 hypothetical protein -
  FEO95_RS18540 (FEO95_18635) - 4033670..4034419 (-) 750 WP_006389071.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37767.44 Da        Isoelectric Point: 4.4089

>NTDB_id=364386 FEO95_RS18525 WP_006388916.1 4028758..4029816(-) (pilM) [Stenotrophomonas maltophilia strain sm-RA9]
MGLIPKSQSPLVGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAMNRSGSKAKLAAAA
VAGSAVITKVIPMPAELDENDMEAQIELEAVNYIPYPIEEVNLDFEVIGAIPNNPEMVQVLLAASRSENVELRQSALELG
GLQAKVMDVEAFAVENAYALVASELPVSIEGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAVLGGLPEMVEEQLGVPTVIANPLA
QMTLGPKVNAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=364386 FEO95_RS18525 WP_006388916.1 4028758..4029816(-) (pilM) [Stenotrophomonas maltophilia strain sm-RA9]
GTGGGGCTCATCCCAAAAAGTCAGTCGCCGCTTGTAGGCGTCGACATCAGTTCGACTGCGGTAAAGCTTTTGCAGCTGTC
CCGCAGTGGCAACCGTTTTCGTGTGGAACATTACGCTGTGGAACCTCTGCCGCCGAACGCGGTGGTGGAGAAGAACATCG
TGGAAGTGGAGGCCGTGGGTGAGGCCATCCGCCGTGCGATGAACCGCTCGGGGAGCAAGGCCAAGCTGGCCGCTGCCGCC
GTCGCTGGGTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGAGCTCGACGAAAACGACATGGAAGCCCAGATCGA
GCTGGAAGCGGTCAACTACATTCCGTACCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGGCGATCCCGAACA
ACCCGGAGATGGTCCAGGTGCTGCTGGCCGCGTCGCGTTCGGAAAACGTGGAACTGCGCCAGTCCGCACTGGAACTGGGT
GGCCTGCAGGCCAAGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTATGCCCTGGTCGCCAGCGAGCTGCCGGT
GTCCATCGAGGGCGTGGTCGCGCTGGTCGACATCGGCGCCACCATGACCACCCTCAATGTCCTGCGCGGTGGCCGCAGCC
TGTACAGCCGCGAACAGGTGTTCGGTGGCAAGCAGCTGACCGACGAGATCATGCGCCGCTACGGCCTGAGCTACGAGGAA
GCGGGCCTGGCCAAGCGCCAGGGCGGGCTGCCGGAAAGCTACGAGATGGAAGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGTTTGCTGCAGTTCTTCTATGCGGGCAGCGAATTCAACCGCGTCGATCACATCGTGCTGGCCGGTG
GCTGCGCCGTGCTCGGTGGCCTGCCGGAAATGGTCGAAGAGCAGCTGGGCGTGCCGACCGTGATCGCCAACCCGCTGGCA
CAGATGACCCTGGGCCCGAAGGTGAACGCACATGCGCTGGCCCAGGATGCCCCCGCGCTGATGATCGCCACCGGTCTGGC
GCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B4STX6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.42

100

0.514
  comM Acinetobacter nosocomialis M2

51.42

100

0.514
  comM Acinetobacter baylyi ADP1

50.284

100

0.503
  pilM Legionella pneumophila strain ERS1305867

47.293

99.716

0.472

Multiple sequence alignment