Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   SMI_RS10375 Genome accession   NC_013853
Coordinates   2114351..2115649 (-) Length   432 a.a.
NCBI ID   WP_000867723.1    Uniprot ID   D3HBP4
Organism   Streptococcus mitis B6     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2109351..2120649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMI_RS10355 (smi_2054) rplI 2110506..2110958 (-) 453 WP_000864219.1 50S ribosomal protein L9 -
  SMI_RS10360 (smi_2055) - 2110955..2112928 (-) 1974 WP_000715137.1 DHH family phosphoesterase -
  SMI_RS10365 (smi_2056) hpf 2113064..2113612 (-) 549 WP_000599100.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SMI_RS10370 (smi_2057) comFC/cflB 2113692..2114354 (-) 663 WP_000649962.1 ComF family protein Machinery gene
  SMI_RS10375 (smi_2058) comFA/cflA 2114351..2115649 (-) 1299 WP_000867723.1 DEAD/DEAH box helicase Machinery gene
  SMI_RS10380 (smi_2059) - 2115706..2116341 (+) 636 WP_000395727.1 YigZ family protein -
  SMI_RS10890 - 2116435..2116530 (+) 96 Protein_2029 PH domain-containing protein -
  SMI_RS10385 (smi_2060) cysK 2116627..2117553 (+) 927 WP_000029885.1 cysteine synthase A -
  SMI_RS10390 (smi_2061) tsf 2117675..2118715 (-) 1041 WP_000808070.1 translation elongation factor Ts -
  SMI_RS10395 (smi_2062) rpsB 2118794..2119573 (-) 780 WP_000268459.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49519.45 Da        Isoelectric Point: 8.3118

>NTDB_id=36349 SMI_RS10375 WP_000867723.1 2114351..2115649(-) (comFA/cflA) [Streptococcus mitis B6]
MKVNPNYLGRLFTENELTKEECQLAEKLPAMRKEKGKLFCQRCDSAILDEWYLPIGAYYCRECLLMKRVRSDQALYYFPQ
EDFPRQDVLKWRGQLTPFQEKVSEELIQAVDKQEPTLVHAVTGAGKTEMIYQVVAKVINGGGAVCLASPRIDVCLELYKR
LQDDFACKISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
DELDRKVRIGELKRLSLPRRFHGNPLIIPKPVWLSDFNHCLEKSRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLKEI
LQEQFPHEKIGFVSSITEDRLEQVQAFRDGKLTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=36349 SMI_RS10375 WP_000867723.1 2114351..2115649(-) (comFA/cflA) [Streptococcus mitis B6]
ATGAAAGTAAATCCAAATTATCTCGGTCGTTTGTTTACTGAGAATGAATTAACTAAAGAGGAATGTCAGTTAGCAGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTGATAGTGCTATTTTAGATGAATGGTATTTGC
CCATCGGTGCTTACTATTGTAGGGAGTGTTTACTGATGAAGCGAGTCAGGAGTGATCAAGCATTATACTATTTCCCACAG
GAGGATTTTCCGAGGCAAGATGTTCTTAAATGGCGTGGTCAATTAACTCCTTTTCAAGAGAAGGTATCAGAGGAACTGAT
TCAAGCAGTAGACAAGCAAGAGCCAACTTTAGTTCATGCGGTGACAGGGGCTGGAAAGACAGAAATGATTTATCAAGTTG
TGGCTAAAGTGATTAATGGAGGTGGTGCAGTTTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAAGATGATTTTGCTTGCAAGATATCTTTACTACACGGAGAATCAGAGCCGTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCCTTTGATTTGCTGATAGTGGATGAAGTAGATGCCTTTCCTTATGTTG
ACAATCCCACGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGCTTGAGAATCTTTTTAACAGCAACTTCTACC
GATGAGTTAGATAGGAAGGTTCGCATCGGAGAATTAAAACGATTGAGTTTGCCAAGACGATTTCATGGAAATCCTTTGAT
TATTCCTAAACCTGTCTGGTTATCCGATTTTAATCACTGTTTAGAGAAAAGTCGGTTGTCACCAAAGCTAAAGTCTTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCATTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAAAAGAAATC
TTACAGGAGCAATTTCCGCATGAGAAAATTGGCTTTGTATCTTCTATCACAGAAGATCGATTAGAGCAGGTACAAGCTTT
TAGAGATGGAAAATTGACAATACTTATCAGTACGACGATATTGGAGCGCGGAGTTACTTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGCCTTTTTACCAAGTCTAGCTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCAACAGGAGATTTGCTTTTTTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCAATTAAGGAAATTCAGCAGATGAA
TAAGGAGGCGGGGCTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(293-396)

Type III restriction enzyme, res subunit (ResIII)

(94-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3HBP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis SK321

94.907

100

0.949
  comFA/cflA Streptococcus mitis NCTC 12261

94.213

100

0.942
  comFA/cflA Streptococcus pneumoniae D39

92.593

100

0.926
  comFA/cflA Streptococcus pneumoniae R6

92.593

100

0.926
  comFA/cflA Streptococcus pneumoniae Rx1

92.593

100

0.926
  comFA/cflA Streptococcus pneumoniae TIGR4

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

50.35

99.306

0.5
  comFA Latilactobacillus sakei subsp. sakei 23K

38.799

100

0.389
  comFA Bacillus subtilis subsp. subtilis str. 168

38.575

94.213

0.363

Multiple sequence alignment