Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GUK18_RS10555 Genome accession   NZ_CP047600
Coordinates   2178689..2180182 (-) Length   497 a.a.
NCBI ID   WP_000249340.1    Uniprot ID   A0A0H2Z2Q5
Organism   Escherichia coli O84:H7 strain Trh52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2173689..2185182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUK18_RS10525 (GUK18_10555) gspK 2174483..2175460 (-) 978 WP_000631648.1 type II secretion system minor pseudopilin GspK -
  GUK18_RS10530 (GUK18_10560) gspJ 2175457..2176062 (-) 606 WP_001350472.1 type II secretion system minor pseudopilin GspJ -
  GUK18_RS10535 (GUK18_10565) gspI 2176059..2176430 (-) 372 WP_000820089.1 type II secretion system minor pseudopilin GspI -
  GUK18_RS10540 (GUK18_10570) gspH 2176427..2176990 (-) 564 WP_016233537.1 type II secretion system minor pseudopilin GspH -
  GUK18_RS10545 (GUK18_10575) gspG 2176994..2177449 (-) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  GUK18_RS10550 (GUK18_10580) gspF 2177466..2178689 (-) 1224 WP_021516462.1 type II secretion system inner membrane protein GspF -
  GUK18_RS10555 (GUK18_10585) pilB/pilB1 2178689..2180182 (-) 1494 WP_000249340.1 type II secretion system ATPase GspE Machinery gene
  GUK18_RS10560 (GUK18_10590) gspD 2180182..2182242 (-) 2061 WP_000498844.1 type II secretion system secretin GspD -
  GUK18_RS10565 (GUK18_10595) gspC 2182272..2183231 (-) 960 WP_024223081.1 type II secretion system protein GspC -
  GUK18_RS10570 (GUK18_10600) gspS2 2183249..2183659 (-) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  GUK18_RS10575 (GUK18_10605) pilD 2183725..2184534 (-) 810 WP_000895857.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55139.25 Da        Isoelectric Point: 5.8815

>NTDB_id=360332 GUK18_RS10555 WP_000249340.1 2178689..2180182(-) (pilB/pilB1) [Escherichia coli O84:H7 strain Trh52]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCSDCRQAAPA
TDEEKRLMGISDTHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=360332 GUK18_RS10555 WP_000249340.1 2178689..2180182(-) (pilB/pilB1) [Escherichia coli O84:H7 strain Trh52]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTACAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGACTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCTACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGATTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCTCCGACTGCCGTCAGGCCGCGCCTGCC
ACCGACGAAGAAAAACGTCTTATGGGGATCTCCGATACGCATGCCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z2Q5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.926

100

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Acinetobacter baumannii D1279779

46.867

80.282

0.376
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Legionella pneumophila strain ERS1305867

43.155

86.72

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

44.75

80.483

0.36